TechNotes Articles

The following articles are from Ambion's TechNotes newsletter. The print version of TechNotes contains additional articles. If you would like to receive a print copy of TechNotes by mail, e-mail us, or give us a call at 1-800-888-8804 (U.S.) or 512-651-0200. For more toll-free phone numbers, see our contact page.

Note: The information contained in each article was current at the time of printing. Since that time, new products that relate to the article may have been released and some products mentioned may have been discontinued.

TechNotes 7:3 (Sep '00)

• Get the 5' End
  Ambion's FirstChoice™ RLM-RACE Kit for selectively amplifying the 5' end in less than a day
  
  
• New Innovations for Southern Blotting
  SouthernMax™ Reagents for Southern Blotting
  
  
• Tools for Blot Hybridization
  Maximize sensitivity, increase data quality, and increase blot lifespans
  
  
• Are Your Solutions RNase-free?
  A foolproof, single-step test for RNase contamination
  
  
• Gene-specific Inactivation by RNA Interference
  The truth about post-transcriptional gene silencing
  
  
• RNAlater Around the World
  RNAlater™ Goes Underground
  
  
• On Target - Answers to Your Frequently Asked Questions
  Absolute quantitation of mRNA species using Northern analysis
  Making probes for nuclease protection assays
  Differences between "linked" and "coupled" transcription:translation systems
  
  
• Tips from the Bench
  Having Trouble Visualizing Your RNA Markers?
  Does Increasing Input Total RNA Affect Yield of RT-PCR Product?
  
  
• Simultaneous Detection of Multiple mRNA Targets
  Multi-gene analysis in a single tube
  
  
• Synthesize mRNA Transcripts in Vitro with More Confidence
  Ambion's mMESSAGE mMACHINE™ is improved with the addition of SUPERase•In™
  
  
• Single Reagent for RNA Isolation
  RNAwiz™: A convenient method for RNA extraction
  
  
• Your Data - Examples of Ambion Products in Everyday Use
  RNA Isolation from Grapefruit for Use in Differential Display
  
  
• Consistent, Fast Gel Preparation
  Prepared acrylamides + urea for nucleic acid analysis
  
  
• RNArticles: Highlighted articles in peer-reviewed journals of interest to RNA researchers
  World's Longest Cloned RNA
  
  

TechNotes 7:2 (Jul '00)

• RIP R.I.P.
  SUPERase•In™ - More flexible than any other RNase inhibitor
  
  
• Ambion Declares War on RNases
  Prevent, detect, and eliminate RNases from all of your experiments
  
  
• The Basics of in Vitro Transcription
  Learn about the requirements for transcription, RNA polymerases, and the differences between large scale and conventional syntheses
  
  
• Examples of Large Scale Transcription Applications
  
  
• Using PCR Products as Templates for Transcription Reactions
  
  
• An Alternative to RT-PCR
  NPAs: A straightforward, yet sensitive method to detect rare mRNAs
  

• RNaseZap® Wipes
  Destroy RNases on contact
  

• RNAlater™ Around the World
  Cell sorter and real-time RT-PCR application
  

• Your Data - Examples of Ambion Products in Everyday Use
  Applications of Ambion's Cells-to-cDNA™ Kit
  

TechNotes 7:1 (Mar '00)

• The Truth: Your RNA Is Contaminated! A New Method to Remove DNA
  Make your RNA ready for RT-PCR with DNA-free
  
  
• The Accuracy of Competitive RT-PCR Depends on Using the Right Exogenous Standard
  Don't waste your time with substandard standards
  
  
• Make Your Data Quantitative: Choosing and Validating Your Internal Control
  Use the proper internal control for your system
  
  
• Simplify RNA Isolation from Plants and Bacteria
  New products that work with your RNA isolation system to make clean RNA
  
  
• RNA Isolation for RT-PCR
  No matter what you start with, Ambion has the tools to get you RT-PCR ready
  
  
• In Situ RNA Detection: Fast, Sensitive Localization of RNA
  Streamline in situ RNA detection with Ambion's mRNAlocator-Hyb and mRNAlocator-Biotin Kits
  
  

TechNotes 6:5 (Dec '99)

• Cells-to-cDNA™ Reverse Transcription without RNA Isolation
  Rapid reverse transcription from cells without RNA isolation
  
  
• The Ultimate RNase Inhibitor: SUPERase•In™
  The most effective RNase inhibitor available
  
  
• Efficient 5' End Selection
  FirstChoice™ RLM-RACE Kit -- Obtain the 5' ends of your messages in less than one day
  
  
• Is Your RNA DNA-free?
  Get rid of contaminating DNA from your RNA with DNA-free
  
  
• Play it Safe...Nuclease Happens!
  New RNaseAlert™ Kit for non-subjective determination of RNase contamination
  
  
• Isolate RNA from Paraffin-Embedded Tissue Blocks
  
  
• How to Isolate RNA for RT-PCR Experiments
  
  

TechNotes 6:4 (Oct '99)

• Get the RNA Out Intact
  Prevent RNA degradation in tissues and cell samples
  
  
• Now Get More Data from Gene Arrays
  Introducing Strip-EZ™ RT Kit, for the synthesis of StripAble™ cDNA probes for gene array analysis
  
  

TechNotes 6:3 (Jun '99)

• Strategies for Detecting mRNA
  A comparison of Northerns, nuclease protection assays, in situ hybridization and RT-PCR for detecting mRNA
  
  
• How to Solve Your RNA Isolation Problems
  
  
• Quantitative RNA Analysis
  How to use NPAs, RT-PCR and Northerns for relative and absolute quantitation of mRNA
  
  
• An Alternative to RT-PCR
  Nuclease protection assay: A straightforward, yet sensitive, method to detect rare mRNAs
  
  

TechNotes 6:2 (Apr '99)

• Get More From Your Blots
  Blot hybridization reagents that increase sensitivity, data quality, and blot lifespan
  
  
• New Hybridization Technology Makes Nonisotopic Probes More Sensitive
  Nonisotopic DNA probes go farther with ULTRAhyb™
  
  

• Making RT-PCR Quantitative
  Solutions to key problems of RT-PCR
  
  
• The Accuracy of Competitive RT-PCR
  Using the right exogenous standard
  
  
• Choose the Right Internal Control
  A discussion of various internal controls, including which are most appropriate for Northerns, NPAs, and RT-PCR
  
  
• Locate Your mRNA
  Ambion’s new in situ hybridization kits
  
  

• Translation: Flexibility AND High Yields
  New gene-to-protein transcription:translation kits
  
  
• In Vitro Translation Basics
  In vitro protein synthesis in eukaryotic and prokaryotic cell-free systems
  
  
• Making RT-PCR Simple
  Achieving linear range easily + the internal control you always wanted
  
  

• Before…RNA Isolation…After
  New ways to handle tissue and store RNA
  
  
• Cell Disruption
  Getting the RNA out
  
  
• Isolating RNA from Difficult Tissue
  
  

• Making the Switch to Nonisotopic RNA Analysis
  
  
• Better Northerns
  Optimized procedure increases sensitivity