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Ambion's TechNotes newsletter is published six times a year and has informative articles on RNA isolation, synthesis, detection and quantitation. Also included are tips to improve your results and details on Ambion's newest products.

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RNAi Resource

The RNAi Resource is an exciting new addition to the Ambion web site, providing you with up-to-the-minute information on developments in siRNA design, execution and analysis. Here, you'll find summaries of important new scientific articles and links to the latest web-based siRNA-related information and design tools.

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect
Tracking labeled siRNA distribution, turnover and strand separation.

Microarray Analysis: Gene Representaton in Amplified vs. Unamplified RNA
Researchers at Corning, Inc. look at gene representation from 1-100,000 cells.

On Target 9(3) - Answers to Your Frequently Asked Questions
Choosing coprecipitants for use with dilute RNA samples and a safer alternative to formaldehyde-based Northerns.

Automating RNA Isolation
Data from 48 samples processed using a 96-well plate based RNA isolation protocol adapted for a robotic liquid handling system.

Cells-to-cDNA II using FACS Sorted Cells
Cells-to-cDNA II Lysis Buffer was used to analyze gene expression of hGUS in FACS sorted Jurkat cells using real-time RT-PCR.

Effect of Freeze-Thawing of Tissue on RNA Integrity
Tips from the Bench

Go From Cells to cDNA Directly: Using FACS Samples
Univ. W. Virginia lab uses cell lysates from FACS processed cells directly for RT-PCR.

Top Ten Sources of RNase Contamination
The Ambion Top Ten

 
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