Cancer-Related Gene Expression Analysis Made Easy with Ambion's Tumor and Normal Adjacent Tissue RNAs

Whether you want to examine gene expression in individual tumors or screen multiple tumors from a given tissue, Ambion has done the hard work for you. We provide high quality total RNA purified from individual human tumors and the corresponding normal adjacent tissue. This RNA is ready for any downstream application including RT-PCR, gene array analysis, Northern analysis, ribonuclease protection assays, differential display or cloning.

Individual Tumor and Normal Adjacent Tissue RNA

FirstChoice™ Human Tumor/Normal Adjacent Tissue RNA pairs include 2 aliquots of 5 µg of total RNA, one isolated from an individual tumor and the other from normal adjacent tissue (NAT) from the same individual. Each product is derived from a single donor and has not been pooled with other RNA, so it can be used for gene expression analysis of a single tumor.

Finest Quality RNA

All of the FirstChoice RNAs are made using the same high quality standards. Each step of the process has been optimized to produce highly pure, intact RNA. To prevent RNA degradation prior to isolation, all tumor/tissue samples are stored in RNAlater™ Tissue Collection:RNA Stabilization Solution. RNAlater quickly permeates tissues and inactivates RNases, so that the RNA is protected immediately upon collection of the tissue, resulting in greater yields of intact RNA. Total RNA is then purified using proprietary techniques that include a rigorous DNase treatment to remove genomic DNA contamination. All Tumor/NAT RNAs are packaged in THE RNA Storage Solution at a convenient, ready-to-use concentration of 0.5 mg/ml. Each of the FirstChoice RNAs is tested for RNase contamination by an overnight incubation at 37ëC followed by agarose gel electrophoresis.