Moving Forward in Reverse Transcription

Whether you need reverse transcription products for cDNA synthesis, or one- or two-step real-time RT-PCR products, Applied Biosystems newest suite of offerings streamlines reactions, providing improved performance, reliability, and efficiency. All Applied Biosystems RT-PCR kits and reagents are optimized for use with either TaqMan Gene Expression Assays or SYBR® Green reagents.

Products for Reverse Transcription
NEW High Capacity RNA-to-cDNA Master Mix
For first-strand cDNA synthesis in high-throughput applications:
• Complete, first-strand cDNA synthesis master mix in a single tube reduces experimental variability and streamlines workflow
• 5X concentration provides robust results even with dilute (<25%) RNA samples and rare or abundant transcripts
• Remains liquid at –10° to –20°C eliminating freeze/thaw cycles and maximizing stability
• Optional “No RT Control” formulation for assay validation
• Convenient 96-well plate format for automated high-throughput applications
[More Information]

NEW High Capacity RNA-to-cDNA Kit
For first-strand cDNA synthesis in low- to medium-throughput applications:
• Complete kit includes 20X Enzyme Mix, containing MuLV RT and RNase Inhibitor Protein, and 2X RT Buffer Mix, containing dNTPs
• Combined components means easy workflow with few pipetting steps
• Short reaction time (0.5–1 h)
• Reliable reverse transcription of both abundant and limited targets
• Optimized 2-step protocol for multiple PCR reactions from a single reverse transcription reaction
[More Information]

Products for Real-Time RT-PCR
TaqMan® RNA-to-CT 2-Step Kit
For high sensitivity, real-time RT-PCR screening of a large number of targets from the same RNA sample:
• A combination of the High Capacity RNA-to-cDNA Reverse Transcription Kit and the TaqMan Gene Expression Master Mix
• Detect as low as 1 copy of target
• Dependable, sensitive, target quantification over a wide dynamic range
• Sensitive for routine and challenging applications such as rare transcript detection, duplex PCR, and specific detection of homologous sequences
• Ideal for duplex PCR, i.e., co-amplifying two targets in a single reaction
• High stability—key for high-throughput handling
• TaqMan Gene Expression Master Mix available separately
[More Information]

Power SYBR Green RNA-to-Ct 2-Step Kit
For rapid, real-time RT-PCR screening
• A combination of the High Capacity RNA-to-cDNA Reverse Transcription Kit and the Power SYBR Green PCR Master Mix
• Detect as few as 2 copies of target
• Sensitive over a broad range of target concentrations
• Contains AmpliTaq Gold® DNA Polymerase, LD, in an optimal formulation to provide greater specificity
• New formulation minimizes variation, ensuring reliable results with every experiment
• Simple substitution for SYBR Green PCR Master Mix in existing protocols
• Power SYBR Green PCR Master Mix available separately
[More Information]

Figure 1. New Applied Biosystems Products for RT-PCR Success.

For more information, visit our website.