Silencer® Pre-designed siRNAs (Ambion)
TaqMan® Gene Expression Assays (Applied Biosystems)
Ensure RNAi Success

Together, Ambion and Applied Biosystems provide a complete and convenient solution for gene silencing experiments. Silencer® siRNAs from Ambion eliminate the guesswork and tedious labwork associated with siRNA design and testing. And gene-specific, ready-to-run TaqMan® Gene Expression Assays from Applied Biosystems give you a rapid and simple way to measure knockdown effectiveness. This successful combination accelerates gene function analyses, enabling you to focus on answering important biological questions rather than on testing and validating reagents.

A simple five-step process uses Applied Biosystems and Ambion reagents to accelerate gene function analysis:

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Figure 1. Five Steps for Successful Gene Function Analysis and the Applied Biosystems and Ambion Reagents and Instruments for Each Step.

• Fast: Ready to use siRNAs for human, mouse, and rat genes

• Easy: Searchable database makes it simple to find siRNAs to your genes of interest

• Guaranteed: Efficient gene knockdown guaranteed

Tired of wasting time with poorly designed siRNAs? Silencer® Pre-designed siRNAs--highly effective, ready-to-use siRNAs available for all human, mouse, and rat genes--provide guaranteed gene silencing. Each siRNA has been designed using a carefully optimized algorithm and then manufactured to exacting standards to provide potent and specific gene silencing.

Journal article reviewers generally require that researchers verify gene silencing results with a second siRNA. Silencer Pre-designed siRNAs make it easy to comply. Ambion guarantees that at least two Silencer Pre-designed siRNAs will knockdown target mRNA levels by ≥70% when three are purchased for the same gene.

Find Silencer Pre-designed siRNAs and corresponding TaqMan® Gene Expression Assays

• Gene-specific TaqMan® probe and primer sets for quantitative gene expression studies in human, mouse, rat, Rhesus, C. elegans, Drosophila, and Arabidopsis

• Convenient single tube format and 20X formulation

• Universal thermal cycling conditions

TaqMan Gene Expression Assays (Figure 2) are a comprehensive collection of more than 600,000 predesigned primer and probe sets that let researchers quickly and easily perform quantitative gene expression studies on human, mouse, rat, Rhesus, C. elegans, Drosophila, and Arabidopsis genes. These assays provide the exquisite sensitivity, linearity, and dynamic range that you have come to expect from  TaqMan chemistry, and all perform under universal thermal cycling conditions. These properties, as well as their availability for the same transcripts to which Ambion Silencer siRNAs are targeted, make TaqMan Gene Expression Assays ideal for analyzing siRNA induced knockdown by qRT-PCR.

Find TaqMan Gene Expression Assays and corresponding Silencer siRNAs for your genes of interest

Figure 2. TaqMan® Gene Expression Assays.

The products listed below are only a small subset of the instruments and reagents available from Ambion for gene silencing research. These products were featured in the accompanying article, Delineating the Role of Survivin in Oncogenesis: An siRNA Study. See www.ambion.com/siRNA for a comprehensive list of kits, reagents and instruments for simplifying your gene silencing research.

Ordering Information for Ambion Products: