| There are two main approaches for inducing RNAi
in mammalian cells: use of siRNAs and use of siRNA expression
vectors. In general, RNAi experiments require positive and negative
control siRNAs or vectors, as well as at least two different
siRNA sequences per target. Independent use of multiple individual
siRNAs per target improves confidence in data and is generally
required for publication of RNAi results.
PRODUCTS
Already designed, guaranteed-to-silence
siRNAs to human, mouse, and rat genes
• Silencer® Pre-designed
siRNAs
• Silencer® Validated
siRNAs
• Silencer® In Vivo
Ready siRNAs
• Silencer® siRNA Libraries
• Silencer® Control
siRNAs
• Silencer® Labeled
Control siRNAs
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Transfection and electroporation are commonly used to
deliver siRNAs into cultured mammalian cells. With both methods,
optimization using positive and negative control siRNAs is
usually required for each cell type used.
PRODUCTS
Transfection
• siPORT™ NeoFX™
• siPORT™ Amine
• siPORT™ Lipid
• Silencer® siRNA Transfection II Kit
• Silencer® CellReady™ siRNA Transfection Optimization Kit
Electroporation
•siPORT™ Electroporation B uffer
• siPORT™ Electroporation Kit
• siPORTer™-96
Electroporation
Chamber
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To assess the effectiveness of an siRNA or
siRNA delivery conditions, knockdown of the target mRNA
and protein should be measured using standard RNA and protein
analysis techniques. The biological effects of silencing a
particular gene can be monitored in a variety of ways, including
cell based assays, reporter gene assays, and
microarrays among others.
PRODUCTS
RNA Isolation
• PARIS™
(Protein and RNA Isolation System)
• RNAqueous® 4PCR Kit
(RNA Analysis)
• Cells-to-Signal™ Kit
(Protein Analysis)
• KDalert™ GAPDH Assay Kit
• Antibodies matched to
Silencer® Control siRNAs
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