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technotes 11(3)
 
   
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Complete Toolkit for Transfection Optimization
NEW—Learn more about kits and reagents that will lead to successful RNA interference experiments.

A New Way to Assess siRNA Delivery Efficiency
NEW—The KDalert™ GAPDH Assay Kit identifies optimal transfection conditions by monitoring siRNA-induced knockdown of GAPDH activity and transfection-induced toxicity.

Research Highlight: Examples of High Throughput siRNA Delivery
A funtional genomic screen identifies kinase genes involved in cell proliferation.

 
 
Getting Started with microRNA Research
An introduction to working with microRNAs

MicroRNA Cloning Overview
A technique is outlined for identifying new microRNAs.

Examine microRNA Profiles from Archived, Formalin-fixed, Paraffin-embedded (FFPE) Tissues
NEW—MicroRNAs isolated with the RecoverAll™ Total Nucleic Acid Isolation Kit can be used in miRNA expression profiling experiments.

 
 


Synthesize High Yields of Biotinylated aRNA
NEW—MessageAmp™ II-Biotin Enhanced includes optimized in vitro transcription and biotin labeling steps to produce enough aRNA for Affymetrix® GeneChips® from just 50 ng total RNA.

Improved Microarray Sensitivity Using Whole Blood RNA Samples
The GLOBINclear™-Human Kit removes up to 95% of globin transcripts before RNA amplification.

Robust High Throughput RNA Isolation from Blood Samples
NEW—MagMAX™-96 Blood purifies quality total RNA from blood or milk samples.

 
 


Recover High Yields of Total Nucleic Acids from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
NEW—RecoverAll™ Total Nucleic Acid Isolation Kit: isolate DNA and RNA (including microRNA).

RNA, DNA, and Protein from a Single Sample
NOW AVAILABLE—TRI Reagent®: a versatile, powerful sample lysis reagent.

Watch Your Tissue MELT into High Quality Total RNA
Multi-Enzymatic Liquefaction of Tissue (MELT™) Total RNA Isolation System disrupts tissues and protects RNA without grinding or polytrons.

RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
Choice of RNA isolation method should account for varying levels of RNase activity (RNaseAlert® Kits).

The Fastest and Most Effective Way to Remove DNA Contamination
TURBO DNA-free™ Kit digests DNA while protecting RNA in a 20 minute procedure.

Your Data: RNAlater® Stories
Preserving RNA in cryosections and studying plant biology in space.

   
 


Jump-start Gene Expression Analyses
FirstChoice® cDNAs are available in two formats: RACE-ready and PCR-ready.

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