pMIR-REPORT™ miRNA Expression Reporter Vector
Sensitive Monitoring of microRNA Activity

• Test sequences for putative miRNA binding sites
• Test interaction between miRNAs and known miRNA binding sites
• Monitor miRNA levels with sensitive, quantitative luciferase activity
• Control for transfection efficiency with ß-galactosidase activity

Figure 1. (A) pMIR-REPORT™ miRNA Expression Reporter Vector. (B) pMIR-REPORT™ ß-Galactosidase Control Vector.

The pMIR-REPORT miRNA Expression Reporter Vector provides an accurate, quantitative, "in cell" measurement of miRNA expression. This validated miRNA reporter system contains a luciferase cDNA under the control of a mammalian promoter/terminator system and a cloning region for putative miRNA binding sites in the 3' UTR (Figure 1A). High luciferase activity indicates little or no functional interaction between the putative miRNA binding site and miRNA in the cell, while low luciferase activity indicates productive interaction between the 3' UTR sequences and miRNA in the cell.

The pMIR-REPORT miRNA Expression Reporter Vector can be used as a screening tool in which random or non-random sequences are inserted into the vector to identify miRNA targets. When coupled with Pre-miR™ miRNA Precursor Molecules, pMIR-REPORT vectors can be used to identify miRNAs that bind to a target sequence of interest. As part of a complete solution, a second vector expressing ß-galactosidase is provided as a control to normalize transfection efficiency (Figure 1B).

pMIR-REPORT miRNA Expression Reporter Vector is the only vector available that has been extensively validated for use as a standard reporter assay for miRNA research. With the pMIR-REPORT vector, you'll be able to quantitate miRNA functional activity.

For an example of how pMIR-REPORT can be used to help identify miRNAs involved in cancer progression, see Cancer-related miRNAs, Uncovered by the mirVana™ miRNA Microarray Platform.

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