Studying miRNAs

Understanding the roles miRNAs play in cellular biology will require a basic understanding of three characteristics: (1) when and where they are expressed, (2) the genes they regulate, and (3) what cellular processes they influence. Ambion has developed products to address each of these questions.

Isolation

1560	mirVana™ miRNA Isolation Kit	Isolate total RNA containing small RNAs; and/or enrich for small RNAs (<200 nt).
1556	mirVana™ PARIS	Isolate protein and RNA from the same sample. Can enrich for RNA <200 nt in length.

Detection

1554	mirVana™ Probe & Marker Kit	End-label small RNA probes and markers
1550	mirVana™ Probe Construction Kit	In vitro transcribe radiolabeled or nonisotopically labeled RNA probes <100 nt in length.
1552	mirVana™ miRNA Detection Kit	Detect attomole (10-18 mol) amounts of target RNA by solution hybridization procedure.

Expression Profiling

1562	NEW mirVana™ miRNA Array Labeling Kit	Label miRNAs with amine NTPs. Compatible with Cy™3 and Cy™5.
1564	NEW mirVana™ miRNA Array Probe Set	Contains probe set for nearly 200 unique human and mouse miRNAs. Compatible with epoxy and aldehyde slides.

See MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers

Functional Studies

17100	NEW Pre-miR™ miRNA Precursors	Introduces or increases specific miRNA expression.
17000	NEW Anti-miR™ miRNA Inhibitors	Reduces specific miRNA activity.

See www.ambion.com/miRNA

siPORT™ Transfection Agents

4503	siPORT™ Amine	Use to transfect small RNAs into cells.
4505	siPORT™ Lipid	Use to transfect small RNAs into cells.
4510	NEW siPORT™ NeoFX™	Use to transfect small RNAs into cells.

See Streamline your siRNA Transfections

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