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TechNotes  10(2)

RNA from Regions of the Human Brain FirstChoice® RNA: Pure, Intact, and Stable

Ambion introduces FirstChoice RNA from distinct regions of the human brain. As The RNA Company®, we are dedicated to providing the highest quality RNA products available. We have worked to develop relationships with a network of accredited organ and tissue procurement organizations to ethically obtain tissues in a manner that preserves RNA integrity. Each RNA preparation is subjected to our stringent Quality Control assays to ensure that the RNA is intact, stable, and free of contaminants, including genomic DNA (<0.05%). This ensures that FirstChoice RNA is ideally suited for any downstream application including array analysis, real time RT-PCR, and Northern analysis. Our expanding list of products includes RNA from frontal cortex, parietal cortex, occipital cortex, basal ganglia, cerebellum, medulla, temporal cortex, and thalamus, as well as over 50 other human tissue types and cell lines and 10 different mouse and rat tissue types and cell lines. Please visit our online catalog for an up-to-date listing and ordering information.

Figure 1. FirstChoice Human Brain Total RNA was stored overnight at either -20°C or 37°C and evaluated on an Agilent 2100 bioanalyzer. The approximate 2:1 ratio of the peaks representing 28S and 18S rRNA indicates that the RNA is intact and stable even when stored at 37°C overnight.

All human tissue samples are acquired through accredited organ/tissue procurement organizations that operate with Institutional Review Board approval and in accordance with Ambion's policies and guidelines including donor consent and protection of privacy.


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