Published siRNA
Target Sequences
April 2003 - The significant increase in
the rate of publication of siRNA target sequences has made it difficult
to maintain a comprehensive list of these sequences. Therefore,
we will no longer be updating this list.
We apologize for any inconvenience
this may cause.
The following siRNA target sequences have been
used in published gene silencing experiments. The extent of silencing,
which was assessed by various methods including immunodetection,
Western blotting, and phenotypic analysis, varied among the targets.
Please see the indicated references for experimental details.
|
Gene (Ref) |
Accession # |
Position from ATG |
Gene Target
(Comments) |
Cell Line Tested
|
siRNA Sequences*
|
Template Sequences**
|
|
ß-actin (1) |
NM_001101 |
972-994 |
aatgaagatcaagatcattgc |
Human HeLa
|
|
|
|
gamma-actin (1) |
NM_001614 |
8-30 |
aagagatcgccgcgctggtca |
Human HeLa
|
|
|
| AIB1
(estrogen receptor coactivator) (22) |
|
|
AAAGACUCCUUAGGACCGCUU |
Ishikawa
|
View, Order |
|
| Apaf-1
(31) |
|
978-998
|
AATTGGTGCACTTTTACGTGA
|
IMR90E1
|
View, Order |
|
|
ARC21 (1) |
AF006086 |
181-203 |
aagaatgaagctgataggacc |
Human HeLa
|
|
|
| ATR
interacting protein (4) |
|
254-274
|
AAGGTCCACAGATTATTAGAT
|
Human HeLa
|
|
|
| ATR
interacting protein (4) |
|
464-484
|
CTAGAGGAACAGAGAAGATCACA
|
Human HeLa
|
|
|
| ATR
interacting protein (4) |
|
867-887
|
AAGAAGAGGCCCAGAAAAGCT
|
Human HeLa
|
|
|
| Bcr-abl
(32) |
|
|
AAGCAGAGUUCAAAAGCCCUU
|
TonB,
K562, hematopoetic cells |
|
|
| Brc-abl
(32) |
|
|
AAAGCAGAGUUCAAAAGCCCU
|
TonB,
K562, hematopoetic cells |
|
|
| beta-tubulin-
mouse neuronal (21) |
AF312873 |
|
AAGACAGAGCCAAGUGGACUC
|
Mouse P19
|
View, Order |
|
| Bruton's
tyrosine kinase (Btk) (33) |
NM_000061 |
1682-1700
|
AAUUGGUAAACGAUCAAGGAG
|
RBL-2H3, WEHI-231, NALM-6
|
View, Order |
|
| Bruton's
tyrosine kinase (Btk) (33) |
NM_000061 |
895-913
|
AAGGGAAAGAAGGAGGUUUCA
|
RBL-2H3, WEHI-231, NALM-6
|
View, Order |
|
| Bruton's
tyrosine kinase (Btk) (33) |
NM_000061 |
518-536
|
AAGAAGCUUAAAACCUGGGAG
|
RBL-2H3, WEHI-231, NALM-6
|
View, Order |
|
| Casein
Kinase I epsilon and delta (CKI-epsilon and CKI-delta) (17) |
|
520-538
|
AACUGGGGAAGAAGGGCAACC
|
HEK293
|
|
|
| Caspase
1 (34) |
|
191-211
|
AAGGGGCACAGGCATGCCAAA
|
IMR90E1A
|
|
|
| Caspase
2 (34) |
|
94-114
|
AAACAGCTGTTGTTGAGCGAA
|
IMR90E1A
|
|
|
| hCdc2
(24) |
|
125-147
|
|
HeLa
|
|
|
| Cdc14A
(23) |
|
89-109
|
GCACAGTAAATACCCACTATT
|
HeLa
|
View, Order |
|
|
cdk1 (1) |
NM_001786 |
125-147 |
aaggggttcctagtactgcaa |
Human HeLa
|
|
|
|
CENP-E (1) |
NM_001813 |
944-966 |
aaactcttactgctctccagt |
Human HeLa
|
|
|
| CHO1
(20) |
|
138-156
|
AAGGUCAGUAAUACAACGGUG
|
CHO
|
|
|
| corin,
mouse (12) |
|
|
AACCACCGCCCUCCGUGCA
|
HL-5 and 293
|
|
|
| Cyclin
B1 (5) |
|
199-217
|
CTCGTACAGCCTTGGGAGACA
|
Xenopus embryo
|
|
|
| Cyclin
B2 (5) |
|
252-270
|
ATGTTAAGACTAAGCATGTAC
|
Xenopus embryo
|
|
|
|
cytoplasmic dynein 1 heavy chain (1) |
U53530 |
509-531 |
aaggccaaggaggcgctggaa |
Human HeLa
|
|
|
| DIP13-alpha
(DCC interacting protein) (25) |
|
|
AAGUGCAGCAACACACCUGAC |
DLD1
|
View, Order |
|
| DIP13-beta
(DCC interacting protein) (25) |
|
|
AAGCAGCACCUCUCCUCCCUU |
DLD1
|
View, Order |
|
Dishevelled
(hDv-1 and hDv-3) (16)
|
|
|
AACAAGATCACCTTCTCCGAG
|
A293
|
|
|
hDv-2
(16)
|
|
|
AACTTTGAGAACATGAGCAAC
|
A293
|
|
|
|
Eg5 (1) |
NM_004523 |
1547-1569 |
aactggatcgtaagaaggcag |
Human HeLa
|
|
|
|
emerin (1) |
NM_000117 |
628-650 |
aaccgtgctcctggggctggg |
Human HeLa
|
|
|
| eve
(11) |
|
|
AACUCCUUGAACGGCAGCCGC
|
Drosophila embryos
|
|
|
| hFbw7
(cyclin E ubiquitination) (26) |
|
713-735
|
|
HeLa
|
|
|
| Fortilin
(35) |
|
121-141
|
AAGGUAACAUUGAUGACUCGC
|
U2OS
|
|
|
| GalTII
(galactosyltransferase II) (27) |
|
65-87
|
AAGCACGCGACGCUGGCGCGC |
HeLa
|
View, Order |
|
| GalTII
(galactosyltransferase II) (27) |
|
461-482
|
AAGGCGGACGACGACUCCUUC |
HeLa
|
View, Order |
|
|
GAS41 (1) |
NM_006530 |
327-349 |
aagacctgtaaccctgtatca |
Human HeLa
|
|
|
| Hec-1
(36) |
NM_006101 |
1517-1539
|
|
HeLa-S3
|
|
|
| HPV
E6 (38) |
|
|
AAGAGGUAUAUGACUUUGCUU
|
CaSKi, SiHa, NDF, HCT116
|
|
|
| HPV
E7 (38) |
|
|
AAAGGAGGAUGAAAUAGAUGG
|
CaSKi,
SiHa, NDF, HCT116 |
|
|
| HtrA2
(28) |
|
|
AAAACGGCUCAGGAUUCGUGG |
U2OS
|
View, Order |
|
| HtrA2
(28) |
|
|
AAGGGGAGTTTGTTGTTGCCA |
U2OS
|
View, Order |
|
| HtrA2
(28) |
|
|
AAGCTTTCCCGATGTTCAGCA |
|
View, Order |
|
|
keratin 18 (1) |
NM_000224 |
1154-1176 |
aagatggcgaggactttaatc |
Human HeLa
|
|
|
| Kinase
interacting stathmin (hKIS) (39) |
|
160-180
|
AAGCAGTTCTTGCCGCCAGGA
|
HEK293
|
|
|
| Kinase
interacting stathmin (mKIS) (39) |
|
160-180
|
AAGCAGTTCCTGCCTCGGGGA
|
HEK293
|
|
|
| lamin
A (13) |
|
260-280
|
GAAGCAGCUUCAGGAUGAGAU
|
Human HeLa S3
|
|
|
| lamin
A (19) |
|
|
AAGGACCUGGAGGUCUGCUG
|
Hep3B
|
|
|
|
lamin A/C (1) |
NM_005572 |
608-630 |
aactggacttccagaagaaca
(90% reduction; 2) |
Human HeLa
|
|
|
|
lamin B1 (1) |
NM_005573 |
672-694 |
aacgcgcttggtagaggtgga |
Human HeLa
|
|
|
|
lamin B2 (1) |
M94362 |
1457-1479 |
aagaggaggaggaagccgagt |
Human HeLa
|
|
|
|
LAP2 (1) |
NM_003276 |
37-59 |
aaagacaagttgaagagtgag |
Human HeLa
|
|
|
| Mad1
(41) |
|
1843-1865
|
|
HeLa-S3
|
|
|
| Mammalian
septin (MSF) (44) |
|
472-492
|
|
HeLa
|
|
|
Methyl-CpG-binding
protein 2 (MeCP2) (19)
|
|
|
AAGCAUGAGCCCGUGCAGCCA
|
Hep3B
|
|
|
| Methyl-CpG
binding domain protein 2 (MBD2) (19) |
|
|
AAGAGGAUGGAUUGCCCGGCC
|
Hep3B
|
|
|
| Microtubule-associated
protein 2 (MAP2) (42) |
|
|
AAUCCUUCGUCUUUCCUCUCG
|
primary neuronal cells
|
|
|
| Myc-induced
nuclear antigen 53 (mina53) (40) |
|
45-63
|
|
HeLa, MycB
|
|
|
| MPS1
kinase (hMps1) (8) |
|
133-155
|
|
Human HeLa S3
|
|
|
| Myeloid
cell leukemia protein 1 (MCL1) (43) |
|
456-476
|
AACCCGUCCGUACUGGUGUUA
|
U2OS
|
|
|
| Nicastrin
(Nct) (45) |
|
|
AAGGGCAAGTTTCCCGTGCAG
|
HEK293
|
|
|
|
NuMA (1) |
Z11583 |
3988-4010 |
aaggcgtggcaggagaagttc |
Human HeLa
|
|
|
|
Nup153 (1) |
NM_005124 |
2593-2615 |
aaggcagactctaccaaatgt |
Human HeLa
|
|
|
| Origin
recognition complex protein 6 (Orc6) (46) |
|
|
AAUGGUAGCCACAUCCGGUGU
|
HeLa
|
|
|
| Origin
recognition complex protein 6 (Orc6) (46) |
|
|
AAGATTGGACAGCAGGUCGAC
|
HeLa
|
|
|
| P160
ROCK (47) |
|
566-584; 639-657; 1958-1976; 2780-2798
|
|
|
|
|
| Polo-like
Kinase1 (hPlk1) (37) |
NM_005030 |
181-203
|
AAGGGCGGCTTTGCCAAGTGC
|
HeLa, U2OS
|
|
|
| Protein
Kinase Receptor (PKR) (9) |
|
931-949
|
AAGATCAAGTTTTGCCAATG
|
Human HeLa
|
|
|
| hRad9
(29) |
|
232-252
|
AAGUCUUUCCUGUCUGUCUUU |
MCF-7
|
View, Order |
|
| Rad17
(7) |
|
|
AACAGACUGGGUUGACCCAUC
|
Human HeLa
|
|
|
| Signalling
Adapter/scaffold protein (h/m Shc) (3) |
|
677-697
|
tcctacttggttcggtacatggg
|
Human HeLa
|
|
|
| p66
ShcA (3) |
|
236-256
|
CGGAATGAGTCTCTGTCATCGTC
|
Human HeLa
|
|
|
Sphingosine
kinase 1 (hSK1) (15)
|
|
70 nt downstream of start codon
|
AAGAGCUGCAAGGCCUUGCCC
|
HUVEC
|
|
|
| Steroid
receptor coactivator (SRC-1) (30) |
|
|
CCUCAGGGCAGAGAACCAUCUTT |
Ishikawa
|
|
|
| ß-tubulin
(6) |
|
3'-UTR
|
GACAGAGCCAAGUGGACUCAC
|
Mouse P19
|
|
|
| Tissue
Factor (hTF) (18) |
|
|
AAGCGCUUCAGGCACUACAAA
|
HeLa, 293, Cos-1, HaCaT
|
|
|
| Tissue
Factor (hTF) (18) |
|
|
AAGAAGCAGACGUACUUGGCA
|
HeLa, 293, Cos-1, HaCaT
|
|
|
| ß-transducin
repeat-containing protein (ß-TrCP), human (10) |
|
|
GUGGAAUUUGUGGAACAUCTT
|
293
|
|
|
| TSG101
(a ubiquitin enzyme 2 variant protein) (14) |
|
413-433
|
AACCUCCAGUCUUCUCUCGUC
|
Human HeLa-M & HeLa KK3
|
|
|
|
VASP (1) |
XM_009141 |
1087-1109 |
aaagaggaaatcattgaagcc |
Human HeLa
|
|
|
|
vimentin (1) |
NM_003380 |
346-368 |
aactacatcgacaaggtgcgc
(No silencing observed; 1,2) |
Human HeLa
|
|
|
|
vimentin (1)
|
NM_003380
|
1145-1167 |
aataccaagacctgctcaatg |
Human HeLa
|
|
|
|
vimentin (1)
|
NM_003380
|
863-885 |
aagaatggtacaaatccaagt |
Human HeLa
|
|
|
|
vimentin (1)
|
NM_003380
|
1037-1059 |
aaatggaagagaactttgccg |
Human HeLa
|
|
|
|
vinculin, mouse (1) |
L18880 |
2923-2945 |
aagcggattagaaccaatctc |
Mouse 3T3
|
|
|
|
zyxin, mouse (1) |
X99063 |
1357-1379 |
|