Products Documents

The types of experiments performed to structurally characterize RNA utilize a variety of reagents that are not readily available from a single source and typically are not optimized for these applications. As The RNA Company, Ambion is making a concerted effort to provide kits, reagents and protocols as a service to the community of scientists that perform these experiments.

As part of this initiative, we have created this RNA Structure/Function Resource, which contains information and links to the most recent articles, protocols and tools for RNA structure/function research available on the Ambion web site.

Tools for RNA Structure/
Function Research
Enzymes
RNA-Grade Ribonucleases
A, V1 and T1


T4 Polynucleotide Kinase

KinaseMax™ 5' End Labeling Kit
(contains T4 Polynucleotide Kinase, CIP, buffers, a control, and a novel CIP removal reagent)

T4 RNA Ligase

Poly(A)Polymerase

In Vitro Transcription Kits
MEGAscript™ - Synthesizes ultra high yields of RNA, 10 to 50 times that produced by conventional transcription reactions.

MEGAshortscript™-
Efficiently transcribes large quantities of RNA from short templates, <300 bases.


MODIscript™ - In vitro transcription kit especially for incorporation of modified nucleotides.

Reference Information

Selected References on RNA Structure/Function Analysis
- Technical Bulletin #504
Includes references (many are comprehensive review articles) on RNA structure analysis, commonly occurring RNA structures and how they are detected, and techniques to analyze protein-RNA interactions
.

Nuclease Characteristics
A handy table of cleavage specificities, molecular weights, pH optima, and required cofactors for commonly used DNase and RNase enzymes.

 


Protocols

Protocol for 5' End Labeling RNA

Protocol for 3' End Labeling RNA

RNA Structure/Function Protocols: Alkaline Hydrolysis, RNA Sequencing and RNA Structure Analyses with Nucleases

Articles

Deciphering Nuclease Digestion Data-
TechNotes 9(2)
A brief description of how to use RNases to define RNA structure

Web Resources

Atlas of nucleic acid structures

Image library of biological macromolecules

Nucleic Acid
Database Project

(an X-ray structure database of nucleic acids and nucleic acid-containing structures)

Nobel prize winners
for structure biology

Biology Workbench
(a suite of online protein and nucleic acid bioinformatics software)

Molecules R Us Utility
(searchable PDB database of protein and nucleic acid structures)

More resources...

 
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