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Technical Bulletins
Hang Up in the Wells -
Preventing Samples from Remaining in the Wells
of Your Gels

TechNotes Articles
Stable Markers for Northern Analysis Markers provided in formamide for increased RNase resistance.

Tips from the Bench 7(3)
Having Trouble Visualizing Your RNA Markers? Does Increasing Input Total RNA Affect Yield of RT-PCR Product?


Ambion's Online Appendix
Gel Electrophoresis Tables
Tables of recommended polyacrylamide and agarose concentrations for separation of different sized nucleic acids and dye migration characteristics in gels of varying polyacrylamide concentrations.


DNA and RNA Molecular
Weights and Conversions

Conversion factors and tables for nucleotide molecular weight, nucleic acid size to molecular weight conversions, nucleotide absorbance maxima, and absorbance units to nucleic acid concentration conversions.


DNA Marker Sizes
This table lists the sizes of commonly used DNA markers, including Lambda DNA cleaved with Hind III, pUC 19 DNA cleaved with Sau3A, and pUC 19 DNA cleaved with Hpa II.

 
RNA Size Markers
Northern Analysis

Millenium Markers™ (0.5 – 9 kb): Millenium Markers are a mixture of 10 discrete RNA transcripts with easy-to-remember sizes: 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, and 9 kb. These increments in marker sizes allow accurate determination of single-stranded RNA transcript sizes across a broad range. Biotinylated and unlabeled markers available can be stained with EtBr either during or after electrophoresis, or they can be end-labeled.

RNA Millenium™ Markers

Millenium™ Markers – Formamide

BrightStar® Biotinylated RNA Millennium™ Markers

Millenium™ Marker Probe Template

Ribonuclease Protection Assays
RNA Century™ Markers (100 – 1000 bases): RNA Century™ Markers are a set of 5 in vitro transcripts of 100, 200, 300, 400 and 500 bases. RNA Century™-Plus Markers are a set of 7 in vitro transcripts of 100, 200, 300, 400, 500, 750 and 1000 bases. The Century Markers and Century Marker Templates are ideal for use in isotopic and non-isotopic ribonuclease protection assays and when single-stranded RNA size standards are needed for denaturing gel electrophoresis.

RNA Century™ Markers
(100 – 500 bases)

RNA Century™-Plus Markers

(100 – 1000 bases)

Century™ Marker Templates

Century™-Plus Marker Templates


BrightStar™ Biotinylated RNA Century™ Markers

(100 – 500 bases)

BrightStar™ Biotinylated RNA Century™-Plus Markers

(100 – 1000 bases)
Small RNA Molecule Analysis
Decade™ Markers (10 – 100 bases): Ambion’s Decade Marker System produces a set of radiolabeled RNA molecules of 100, 90, 80, 70, 60, 50, 40, 30, 20, and 10 nucleotides. The Decade Marker System is particularly useful for research laboratories studying tRNA, snRNA, snoRNA and other small RNA molecules.
RNA 6000 Ladder

The RNA 6000 Ladder is designed for use with the RNA 6000 LabChip® Kits and the Agilent 2100 Bioanalyzer. The Ladder is composed of six RNA transcripts that produce distinct peaks of 0.2, 0.5, 1.0, 2.0, 4.0, and 6.0 kb.

DNA Size Markers

Lambda DNA - Hind III digested
pUC19 DNA - Hpa II digested
pUC19 DNA - Sau3A I digested

 

 
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