Ambion, Inc. - Tested Transfection Conditions for Various Cell Types (siPORT XP-1)

Tested Transfection Conditions Using siPORT™ XP-1 Transfection Agent

Transfection of PCR-derived siRNA expression cassettes (SECs) with siPORT XP-1. SECs were prepared with the Silencer Express (Human U6) siRNA Expression Cassette Kit.
Cell line	DNA construct used	% gene reduction	% transfection efficiency	*siPORT XP-1* (µl)**	DNA (ng)	Cell #/ well	Plate format	Serum?
Cos-7	SEC targeting GAPDH	94		1.2	100	50,000	24-well	Y
DU145	SEC targeting GAPDH	77		2	50	50,000	24-well	Y
HEK 293	SEC targeting GAPDH	90		0.6	100	75,000	24-well	Y
HeLa	SEC targeting GAPDH	95		0.6	200	60,000	24-well	Y
HepG2	SEC targeting GAPDH	68		2	50	60,000	24-well	Y
MCF-7	SEC targeting GAPDH	93		0.6	100	60,000	24-well	Y

Transfection of plasmid DNA with siPORT XP-1.
Cell line	DNA construct used	% gene reduction	% transfection efficiency	*siPORT XP-1* (µl)**	DNA (ng)	Cell #/well	Plate format	Serum?
Cos-7	pSilencer 2.0-U6 targeting GAPDH	91		0.6	200	50,000	24-well	Y
DU145	pSilencer 2.0-U6 targeting GAPDH	93		1.2	200	50,000	24-well	Y
HEK 293	GFP expression plasmid		60-80%	0.6	200	75,000	24-well	Y
HeLa	pSilencer 2.0-U6 targeting GAPDH	90		0.6	200	60,000	24-well	Y
HepG2	pSilencer 2.0-U6 targeting GAPDH	60		0.6	200	60,000	24-well	Y
MCF-7	pSilencer 2.0-U6 targeting GAPDH	56		0.6	200	60,000	24-well	Y