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A highly predictive siRNA design algorithm and stringent specificity
checking are the keys to effective siRNA design. Ambion, through
its partnership with Cenix BioScience, provides Silencer siRNAs — ready-to-use
siRNAs designed for maximum potency and specificity using the most
extensively tested siRNA design algorithm in the industry.
Although several web-based siRNA design tools are now available,
almost none provide statistics as to the success rate of the underlying
algorithm, as few have been rigorously tested. The few algorithms
that have been analyzed have typically been tested using siRNAs to
very few targets. Based on extensive tests performed at both Ambion
and Cenix, it has become clear that small test sets do not accurately
reflect the success rate of an algorithm, as some targets are more
difficult to silence than others. The algorithm used to design Ambion's
Silencer siRNAs has been functionally tested using >1100 siRNAs
to nearly 400 endogenous human targets. This large data set (Figure
below) shows that the algorithm successfully predicts effective siRNAs.
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