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| Survivin’s Role in Cell Survival | ||
| NFkB Pathway Analysis | ||
| Assessing the Roles of 178 Kinases in the Cell Cycle | ||
RNAi Experimental Steps 1. Order Silencer Validated siRNA to Survivin at www.ambion.com/siRNA (siRNA ID: 42862)2. Transfect HeLa cells with 30 nM Survivin siRNA or Silencer Negative Control #1 siRNA in quadruplicate 3. Incubate 48 hoursIsolate RNA and analyze survivin mRNA levels by real-time PCR (duplicates) 4. Stain nuclei with DAPI and analyze nuclear morphology by fluorescence microscopy (duplicates) |
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| Conclusion: Knockdown of survivin by 80% in HeLa cells leads to nuclear morphology changes consistent with apoptosis | ||