Expressing siRNAs in Mammalian Cells
This lecture reviews the use of DNA based vectors (plasmids, PCR products and viral vectors) for the expression of siRNAs in vivo. Examples of RNAi are provided for each method.

Involvement of microRNAs in Cancer and Brain Development
This presentation provides background information on miRNAs, describes tools to study them, and gives examples of the links between miRNA expression and cancer and miRNA expression and neuron differentiation.

Isolation & Detection of Small RNA Molecules (siRNAs, miRNAs)
This lecture describes a method for isolation of small RNA molecules, including an enrichment procedure that makes their detection easier. It also describes a solution hybridization assay for detection of small RNAs as well as mRNAs and provides examples of the detection of miRNA, siRNA, and mRNA individually and simultaneously. Probe synthesis and labeling is also addressed.

MELT™–A Novel Method for Nucleic Acid Preservation and Isolation from Solid Tissue
This presentation details the development of the MELT technology, the experiments that evaluated the yield, intactness, and purity of the isolated RNA, and the suitability of this RNA for gene expression profiling. A new innovation in the MELT protocol, namely the isolation of genomic DNA from MELT tissue lysates, is also described.

RNAi Basics and siRNA Experimental Design
Here we discuss factors involved in good siRNA design and the development of an algorithm that yields highly effective and potent siRNAs. Proper experimental controls and case studies are presented.

RNAi Goes Genomic: Elucidating Gene Function with siRNA Libraries
High efficacy siRNA libraries provided in small aliquots and new methods for high throughput siRNA delivery now make it possible for any lab to perform RNAi screening experiments. This presentation describes experimental set-up and provides examples of screening data to demonstrate the utility of this technique.

Six Methods for Inducing RNAi in Mammalian Cells
This lecture describes 3 methods to synthesize siRNAs in vitro, and 3 methods for expressing them from DNA vectors in vivo. We provide examples of RNAi with each, discuss their advantages and disadvantages, and suggest why you might choose one method of siRNA preparation over another.

Solving siRNA Delivery Problems
This presentation describes three different siRNA delivery techniques (transfection, electroporation, and infection). Applications of these methods are presented along with analyses of how these methods impact siRNA efficacy and specificity.