| pSilencer™ 4.1-CMV puro |
| Vector Map • Promoter/Cloning Site Region • Restriction Sites • Text-Based Sequence |
| pSilencer 4.1-CMV puro Vector Map |
 |
This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (55 base pairs) between the BamH I and Hind III sites. |
|
pSilencer 4.1-CMV
puro Annotated Promoter/Cloning Site Region |
 |
| pSilencer 4.1-CMV puro Restriction Sites |
| Enzymes with single Restriction Sites | Non-Cutting Enzymes : |
||
| Site | Enzyme | Enzyme | |
|
1110
|
Acc65I
|
AarI | |
|
4245
|
AccI
|
AfeI | |
|
1574
|
AflIII
|
AflII | |
|
4413
|
AgeI
|
AleI | |
|
2467
|
AhdI
|
ApaI | |
|
539
|
AloI
|
AscI | |
|
507
|
AloI'
|
AsiSI | |
|
1990
|
AlwNI
|
BaeI | |
|
4464
|
AvrII
|
BaeI' | |
|
515
|
BamHI
|
BbsI | |
|
1104
|
BglII
|
BbvCI | |
|
763
|
BsaAI
|
BclI | |
|
3629
|
BsaBI
|
BlpI | |
|
4296
|
BsiWI
|
BmgBI | |
|
3542
|
BsmI
|
Bpu10I | |
|
4439
|
BspMI
|
BsgI | |
|
3897
|
BssHII
|
BspEI | |
|
4217
|
BstEII
|
BsrGI | |
|
3788
|
DraIII
|
BstBI | |
|
1118
|
EcoRI
|
BstXI | |
|
462
|
HindIII
|
BstZ17I | |
|
3528
|
HpaI
|
Bsu36I | |
|
1114
|
KpnI
|
ClaI | |
|
3537
|
MfeI
|
EcoICRI | |
|
3981
|
MscI
|
EcoNI | |
|
406
|
NotI
|
EcoO109I | |
|
1574
|
PciI
|
EcoRV | |
|
3508
|
PsiI
|
FalI | |
|
4436
|
PstI
|
FalI' | |
|
4237
|
RsrII
|
FseI | |
|
4144
|
SacII
|
FspAI | |
|
4244
|
SalI
|
MluI | |
|
1458
|
SapI
|
NaeI | |
|
4436
|
SbfI
|
NgoMIV | |
|
2947
|
ScaI
|
NruI | |
|
4518
|
SfiI
|
PacI | |
|
763
|
SnaBI
|
PflMI | |
|
3271
|
SspI
|
PmeI | |
|
4313
|
Tth111I
|
PmlI | |
|
3636
|
XbaI
|
PpuMI | |
|
3066
|
XmnI
|
PshAI | |
| PspOMI | |||
| PsrI | |||
| PsrI' | |||
| SacI | |||
| SanDI | |||
| SgrAI | |||
| SpeI | |||
| SrfI | |||
| SwaI | |||
| TaqII' | |||
| XcmI | |||
| XhoI | |||
|
||
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