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| Technical
Resources > Product Info. > Vector Maps |
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| pSilencer™ 3.1-H1
neo Vector Map |
This map and the annotated sequence below
portrays the circular negative control vector provided with the kit.
It includes the negative control siRNA template (66 base pairs) between
the BamH I and Hind III sites.
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pSilencer™ 3.1-H1
neo Annotated Promoter/Cloning Site Region
We recommend the following primers for sequencing:
M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'
3.0rev: 5'-GAGTTAGCTCACTCATTAGGC-3'
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pSilencer™ 3.1-H1
neo Restriction Sites
Vector Size: 4285 bp |
| Enzymes
with single Restriction Sites |
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Non-Cutting Enzymes :
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| Site |
Enzyme |
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Enzyme |
| 4220 |
AatII |
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AarI |
| 915 |
AflIII |
|
Acc65I |
| 1808 |
AhdI |
|
AccI |
| 1331 |
AlwNI |
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AfeI |
| 3772 |
AvaI |
|
AflII |
| 3793 |
AvrII |
|
AgeI |
| 495 |
BamHI |
|
AleI |
| 3226 |
BanII |
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AloI |
| 2313 |
BcgI |
|
AloI' |
| 2347 |
BcgI' |
|
ApaI |
| 3742 |
BclI |
|
AscI |
| 3283 |
BsaAI |
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AsiSI |
| 3728 |
BsaBI |
|
BaeI |
| 790 |
BsaXI |
|
BaeI' |
| 760 |
BsaXI' |
|
BbsI |
| 3803 |
BseRI |
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BbvCI |
| 45 |
BsmBI |
|
BglII |
| 2667 |
BsmI |
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BlpI |
| 3186 |
BssHII |
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BmgBI |
| 3677 |
EagI |
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BmtI |
| 4274 |
EcoO109I |
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Bpu10I |
| 396 |
EcoRI |
|
BsgI |
| 556 |
HindIII |
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BsiWI |
| 3505 |
MscI |
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BspEI |
| 184 |
NdeI |
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BsrGI |
| 2903 |
NruI |
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BstBI |
| 915 |
PciI |
|
BstEII |
| 3538 |
PstI |
|
BstXI |
| 3068 |
RsrII |
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BstZ17I |
| 2288 |
ScaI |
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Bsu36I |
| 4025 |
SexAI |
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BtrI |
| 3774 |
SmaI |
|
ClaI |
| 3798 |
StuI |
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DraIII |
| 3468 |
Tth111I |
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EcoICRI |
| 3772 |
XmaI |
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EcoNI |
| 2407 |
XmnI |
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EcoRV |
| 4218 |
ZraI |
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FseI |
| |
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FspAI |
| |
HincII |
| |
HpaI |
| |
KpnI |
| |
MfeI |
| |
MluI |
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NheI |
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NotI |
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PacI |
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PflMI |
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PmeI |
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PmlI |
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PpuMI |
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PshAI |
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PspOMI |
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PsrI |
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PsrI' |
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SacI |
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SacII |
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SalI |
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SanDI |
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SbfI |
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SfiI |
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SgfI |
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SgrAI |
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SnaBI |
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SpeI |
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SrfI |
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SwaI |
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TaqII' |
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XbaI |
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XcmI |
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XhoI |
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Applied Biosystems. All rights reserved. |
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