Once the target sequence of your siRNA has been chosen, use this tool to determine the sequences of the sense and antisense siRNA oligonucleotide templates for use with the Silencer siRNA Construction Kit. The program will add "CCTGTCTC" (complementary to the T7 Promoter Primer supplied in the kit) to the 3' end of each oligonucleotide, as outlined in section 3a (p. 7) of the kit's Instruction Manual.

See the Instruction Manual or "siRNA Design Guidelines" for additional information on selecting siRNA target sites.