Products Documents
Which RNA Isolation Kit to Choose?
RNA Type
Organism/ Sample Type
Specialized Application
Product
Disruption/ Separation Method
Amount of Starting Material
(per Rxn)
No. of Rxns
Mammalian Cells/Tissue
MELT Tissue Lysis Solution/Magnetic Beads
Up to 10 mg tissue
50
RNAqueous®
Guanidinium thiocyanate/ Glass fiber filter
1–75 mg tissue or 102–107 cells
50
100–500 mg tissue or 107–108 cells
15
Up to 10 mg tissue or 105 cells
50
TRI Reagent®/ Glass fiber filter
5–100 mg tissue
105–106 cells
50
Single-step disruption/ separation reagent
>5 mg tissue or >105 cells
Variable
Guanidinium thiocyanate/ Phenol:chloroform
Up to 10 g tissue or 109 cells
Variable
PCR
0.5–75 mg tissue or 102–107 cells
30
Isolation of both RNA & protein
0.5–75 mg tissue or 102–107 cells
50
Small RNA analysis
0.5–250 mg tissue or 102–107 cells
20-40
0.5–100 mg tissue or 102–107 cells
20-40
Isolation from fixed (FFPE) tissue
Four 20 µm sections
40
LCM
Up to 10 mg tissue or 105 cells
50
Array analysis
10–100 mg tissue or 106 cells
96
High throughput/
Automated
0.1–1.5 mg tissue or 102–106 cells
192
0.1–1 mg tissue or 102–105 cells
384
MagMAX™-96
Up to 10 mg tissue or 102–105 cells
96
Bacteria
Bacteria only
108–1010 cells
50
Mixture of bacterial and mammalian RNA
Mixed RNA samples containing 5–100 µg host RNA
5-20
Blood
0.3–0.5 ml

0.1–0.5 ml
40

25
Isolate RNA from leukocytes
9–10 ml
20
High throughput
50 µl blood or milk
96
Remove globin mRNA from total RNA
1–10 µg total RNA
20
Plants
75–250 mg tissue
40-125
Virus
Samples up to 400 µl containing as few as 20 copies of virus
50
High throughput/
Automated
Samples up to 50 µl containing as few as 50 copies of virus
96
Yeast
Up to 3 x 108 cells
50
Mammalian Cells/Tissues
0.2–2 mg total RNA
6
2–400 µg total RNA
20
30 µg to 8 mg total RNA
Variable
Bacteria
2–10 µg total RNA
20

Updated 05/06

 
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