|
|
Organism/ Sample Type |
Specialized Application |
|
Disruption/ Separation
Method |
Amount of Starting Material (per Rxn)
|
|
|
|
Mammalian
Cells/Tissue |
|
|
MELT Tissue Lysis Solution/Magnetic Beads |
Up to 10 mg tissue |
50 |
| RNAqueous® |
Guanidinium thiocyanate/ Glass fiber filter |
1–75 mg tissue or 102–107 cells |
50 |
|
100–500
mg tissue or
107–108 cells |
15 |
|
Up to 10 mg tissue or 105 cells |
50 |
|
TRI Reagent®/
Glass fiber filter |
5–100
mg tissue
105–106 cells |
50 |
|
Single-step
disruption/ separation reagent |
>5 mg tissue or >105 cells |
Variable |
|
Guanidinium
thiocyanate/ Phenol:chloroform |
Up
to 10 g tissue or 109 cells |
Variable |
PCR |
|
0.5–75
mg tissue or 102–107 cells |
30 |
Isolation
of both RNA & protein |
|
0.5–75
mg tissue or 102–107 cells |
50 |
Small RNA analysis |
|
0.5–250
mg tissue or 102–107 cells |
20-40 |
|
0.5–100
mg tissue or 102–107 cells |
20-40 |
Isolation from fixed (FFPE) tissue |
|
Four 20 µm sections |
40 |
LCM |
|
Up
to 10 mg tissue or 105 cells |
50 |
Array analysis |
|
10–100
mg tissue or 106 cells |
96 |
High throughput/
Automated |
|
0.1–1.5
mg tissue or 102–106 cells |
192 |
|
0.1–1
mg tissue or 102–105 cells |
384 |
| MagMAX™-96 |
Up
to 10 mg tissue or 102–105 cells |
96 |
Bacteria |
Bacteria only |
|
108–1010 cells |
50 |
Mixture of bacterial
and mammalian RNA |
|
Mixed
RNA samples containing 5–100 µg host RNA |
5-20 |
Blood |
|
|
0.3–0.5
ml
0.1–0.5 ml
|
40
25
|
| Isolate RNA from leukocytes |
|
9–10 ml |
20 |
| High throughput |
|
50 µl blood or milk |
96 |
| Remove globin mRNA from total RNA |
|
1–10 µg total RNA |
20 |
Plants |
|
|
75–250
mg tissue |
40-125 |
Virus |
|
|
Samples
up to 400 µl containing as few as 20 copies of virus |
50 |
High throughput/
Automated |
|
Samples
up to 50 µl containing as few as 50 copies of virus |
96 |
Yeast |
|
|
Up
to 3 x 108 cells |
50 |
|
|
Mammalian
Cells/Tissues |
|
|
0.2–2 mg total RNA
|
6 |
|
2–400 µg
total RNA |
20 |
|
30
µg to 8
mg total RNA |
Variable |
Bacteria |
|
|
2–10 µg
total RNA |
20 |
Updated
05/06 |