| Probe Type |
Application |
Starting Material for Probe
Synthesis |
Probe Synthesis Reaction |
Ambion Kit |
Comments |
| dsDNA (I,N) |
Northern, Southern, dot blots |
cDNA inserts, PCR product,
plasmid with insert |
Random priming reaction |
DECAprime™ II |
- Fastest reaction
- Least sensitive
|
| ssRNA
(I,N) |
NPA,
in situ hybridization |
Plasmid
with insert and polymerase promoter |
In vitro
transcription |
MAXIscript™ |
- For use in ribonuclease protection assay
|
| Oligonucleotide
(I) |
Northern,
Southern, dot blots |
Oligonucleotide |
End labeling |
KinaseMax™ |
- Useful when sequence information is limited
|
| NPA |
Oligonucleotide |
End labeling |
KinaseMax™ |
- Useful only for abundant messages
|
ssRNA
ssDNA
dsDNA
Oligonucleotide (N) |
Northern,
Southern, Dot blots, in situ hybridization |
DNA, RNA
or oligonucleotide |
Chemical
labeling, post-nucleic acid synthesis |
BrightStar™ Psoralen-Biotin |
- Sensitivity depends on nucleic acid labeled (e.g., ssRNA
vs. dsDNA)
|