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Markers & Electrophoresis Reagents

• Hang Up in the Wells - Preventing Samples from Remaining in the Wells of Your Gels (Technical Bulletin #163)
  
  

Northern Analysis

• Use of Internal and External Standards or Reference RNAs for Accurate Quantitation of RNA Levels (Technical Bulletin #151)
  
  
• Increasing Sensitivity in Northern Analysis with RNA Probes (Technical Bulletin #168)
  
  
• Membrane Transfer and Crosslinking for RNA (Technical Bulletin #169)
  
  
• Practical Tips for Optimal Northern Analysis (Technical Bulletin #179)
  
  
• Analysis of Differential Gene Expression (Technical Bulletin #500)
  
  

Nuclease Control

• Working with RNA (Technical Bulletin #159)
  
  
• Nuclease and Protease Testing (Technical Bulletin #166)
  
  
• RNase and DEPC Treatment: Fact or Laboratory Myth? (Technical Bulletin #178)
  
  
• How to Maintain an RNase-free Lab (Technical Bulletin #180)
  
  

Probe Labeling Systems

• Use of Internal and External Standards or Reference RNAs for Accurate Quantitation of RNA Levels (Technical Bulletin #151)
  
  
• In Vitro Synthesis of RNA (Technical Bulletin #156)
  
  
• Maximize DNA Probe Sensitivity (Technical Bulletin #157)
  
  
• Probe Specific Activity (Technical Bulletin #162)
  
  
• Increasing Sensitivity in Northern Analysis with RNA Probes (Technical Bulletin #168)
  
  
• When Labeled Nucleotides Go Bad (Technical Bulletin #170)
  
  
• Gel Purification of Probes for Nuclease Protection Assays (Technical Bulletin #171)
  
  
• Ambion's BrightStar™ Nonisotopic Detection System: Tips for Minimizing Background (Technical Bulletin #172)
  
  
• Methods for Nonisotopic Labeling of RNA by in Vitro Transcription (Technical Bulletin #173)
  
  
• Determining RNA Probe Specific Activity and Yield (Technical Bulletin #174)
  
  
• BrightStar™ Psoralen-Biotin Labeled Probes (Technical Bulletin #175)
  
  
• Practical Tips for In Vitro Transcription (Technical Bulletin #182)
  
  
• Practical Tips for Nonisotopic Detection (Technical Bulletin #184)
  
  
• The Basics of In Vitro Transcription (Technical Bulletin #186)
  
  

RNA Isolation

• Application of Prepared Liquid Phenols (Technical Bulletin #158)
  
  
• Working with RNA (Technical Bulletin #159)
  
  
• The Use of LiCl Precipitation for RNA Purification (Technical Bulletin #160)
  
  
• Simultaneous Extraction of RNA and DNA (Technical Bulletin #161)
  
  
• Avoiding DNA Contamination in RT-PCR (Technical Bulletin #176)
  
  
• Isolation of Total RNA from Difficult Tissues (Technical Bulletin #177)
  
  
• Methods to Remove DNA Contamination from RNA Samples (Technical Bulletin #181)
  
  
• Cell Disruption: Getting the RNA Out (Technical Bulletin #183)
  
  

RNA Structure/Function

• Selected References on RNA Structure/Function Analysis (Technical Bulletin #504)
  
  

RNAi/siRNA

• Published siRNA Target Sequences (Technical Bulletin #502)
  
  
• siRNA Design Guidelines (Technical Bulletin #506)
  
  

RT-PCR

• Use of Internal and External Standards or Reference RNAs for Accurate Quantitation of RNA Levels (Technical Bulletin #151)
  
  
• Avoiding DNA Contamination in RT-PCR (Technical Bulletin #176)
  
  
• Methods to Remove DNA Contamination from RNA Samples (Technical Bulletin #181)
  
  
• The Accuracy of Competitive RT-PCR: Using the Right Exogenous Standard (Technical Bulletin #185)
  
  
• Analysis of Differential Gene Expression (Technical Bulletin #500)
  
  

Ribonuclease Protection Assays

• Use of Internal and External Standards or Reference RNAs for Accurate Quantitation of RNA Levels (Technical Bulletin #151)
  
  
• Choice of Ribonucleases for Ribonuclease Protection Assays (Technical Bulletin #152)
  
  
• Simultaneous Detection of Multiple mRNA Targets with Ribonuclease Protection Assays (Technical Bulletin #155)
  
  
• Hang Up in the Wells - Preventing Samples from Remaining in the Wells of Your Gels (Technical Bulletin #163)
  
  
• Absolute Quantitation of mRNA Species Using Northern Blotting, Nuclease Protection Assays and RT-PCR (Technical Bulletin #165)
  
  
• Optimal Probe Concentrations for Ambion's Ribonuclease Protection Assay Kits (Technical Bulletin #167)
  
  
• Gel Purification of Probes for Nuclease Protection Assays (Technical Bulletin #171)
  
  
• Analysis of Differential Gene Expression (Technical Bulletin #500)
  
  
• Linearity of RNase Protection Assays (Technical Bulletin #501)
  
  

Transcription (Large Scale)

• In Vitro Synthesis of RNA (Technical Bulletin #156)
  
  
• Practical Tips for In Vitro Transcription (Technical Bulletin #182)
  
  
• The Basics of In Vitro Transcription (Technical Bulletin #186)
  
  

Transcriptome Analysis

• Selected References on Microarray Analysis (Technical Bulletin #505)
  
  

Translation Systems

microRNA Studies