Agarose Gel Electrophoresis of RNA
A protocol for denaturing gel electrophoresis of RNA for Northern blot or quality assessment. Native gel electrophoresis is also described.
Alternative Protocol for Extraction of RNA from Cells Captured on LeukoLOCK™ Filters Using TRI Reagent®
Blood Fractionation Protocol for Collection of White Blood Cells
This protocol describes the fractionation of 2.5-10 ml blood samples for the collection of WBCs for subsequent storage and/or RNA isolation.
Care and Handling of siRNA Oligonucleotides
General handling and storage instructions, and directions on how to resuspend and anneal siRNA oligonucleotides.
Determining Exponential Range in PCR
The exponential phase of amplification occurs in the PCR cycles when reaction components are still in excess and the PCR products are accumulating at a constant rate. This short article explains how to determine where the exponential phase occurs during PCR.
Fluorescently Labeling Long dsRNA with the Silencer™ siRNA Labeling Kit
A brief protocol for labeling long dsRNA for RNAi research using the Silencer siRNA Labeling Kits.
Gel Purification of miRNA from Total RNA
Genomic DNA Preparation from RNAlater™ Preserved Tissues
A protocol for isolating genomic DNA from tissues preserved in Ambion's RNAlater Tissue Storage:RNA Stabilization Solution.
Isolating Small RNAs
Using the MagMAX™-96 for Microarrays Kit
With very slight modifications, the MagMAX-96 for Microarrays Kit can be used to isolate total RNA that includes the small RNA fraction (miRNA, tRNA, 5S/5.8S rRNA, and other small RNA species).
Isolation of Small RNAs
Using the RiboPure™-Blood Kit
With a few additional reagents and significant protocol modifications, the RiboPure™-Blood Kit can be used to isolate total RNA that includes the small RNA fraction from RNAlater® Solution-treated human blood samples.
Miniprep for Isolating Transcription-quality Plasmid DNA
This miniprep protocol yields high quality template DNA for increased yields following transcription reactions.
Protocol for 3' End Labeling RNA
A protocol for 3' end labeling RNA using T4 RNA ligase and [32P]pCp.
Protocol for 5' End Labeling RNA
This protocol is for 5' end labeling RNA with T4 polynucleotide kinase (PNK).
Protocol for Synthesizing Biotinylated aRNA
Using MEGAscript™ High Yield in Vitro Transcription Kit
Protocol for Trichloroacetic Acid (TCA) Precipitation
A protocol for monitoring the efficiency of radiolabeled nucleotide incorporation in a polymerization reaction by TCA precipitation.
Quantitating First Strand cDNA Using OliGreen
This protocol provides a nonradioactive method for cDNA quantitation using OliGreen® ssDNA quantitation reagent (Molecular Probes) and a standard curve of random decamers.
RNA Structure/Function Protocols: Alkaline Hydrolysis, RNA Sequencing and RNA Structure Analyses with Nucleases
How to use RNases A, T1 and V1 to provide information about the structure of an RNA molecule. Includes two protocols for preparing RNA "ladders" for analysis by gel electrophoresis.
RNAqueous™-96 RNA Isolation Kit Automation Protocol
An automated RNAqueous-96 protocol developed for the MultiPROBE® II HT Liquid Handling System with a Gripper Integration Platform. The protocol should be adaptable to other liquid handling systems.
SDS-Polyacrylamide Mini-gel Recipes
How to prepare sample buffer, stacking gel, resolving gel, and running buffer for SDS-polyacrylamide mini-gel electrophoresis.
Sodium Acetate Precipitation of Small Nucleic Acids
This precipitation protocol can be used to concentrate small nucleic acids from dilute solutions.
TRI Reagent® DNA/Protein Isolation Protocol
Describes the isolation of DNA and protein from the interphase and organic/phenol phase produced during the lysate extraction step of the TRI Reagent® RNA isolation protocol.
Using PharMingen RiboQuant® Multiprobe Template with RPA III™ and MAXIscript™ Kits
PharMingen’s RiboQuant™ multiprobe template sets work well with Ambion's RPA III and MAXIscript Kits — with a few modifications to the standard protocols. Those modifications are outlined in this PDF document.