Screening Guide Request Form
Thank you for your interest in Ambion's siRNA Screening Application Guide! This guide provides an in-depth look into the ways and means of performing siRNA library screens in cultured cells. Inside you will find chapters on experimental design, optimizing transfection in 96- and 384-well plates, assay optimization, and data analysis. Here are the chapter titles:
Chapter 1. Overview of RNA Interference
Chapter 2: RNAi Screening Overview
Chapter 3: Experimental Design: Choosing siRNAs and Cells
Chapter 4: siRNA Delivery Optimization
Chapter 5: Assay Development for siRNA Screening
Chapter 6: Conducting Screening Experiments and Initial Data Analysis
Chapter 7: Validation of Screening Results
Chapter 8: Reagents for siRNA Screening
To obtain a free copy of the siRNA Screening Application Guide—a $40 value—simply answer the five questions below.
1.
Does your lab currently use siRNA libraries (sets of 50 or more siRNAs) for performing either large or small RNAi screens?
Yes.
No, but I plan to within the next 6 months.
No, but I plan to within the next 12 months.
No, and I do not plan to within the next year.
2.
What model system do you use in your RNAi experiments?
Human
Mouse
Rat
C. elegans
Drosophila
Other. Please specify:
None. I am not performing RNAi experiments.
3.
In what types of siRNA libraries are you most interested?
Entire genome siRNA libraries
Druggable genome siRNA libraries
Libraries based on gene function (e.g., kinases, GPCRs)
Collections based on a biological pathway (e.g., apoptosis)
Custom collections
None. I am not interested in siRNA libraries.
4.
Are you interested in learning more about Ambion's siRNA libraries?
Yes
No
5.
What general techniques do you use or plan to use in your lab?
Automation/High throughput
Gene expression analysis
In situ hybridization/Tissue microarrays
In vitro translation
Laser capture microdissection
Microarray analysis
microRNA
Northern blotting
Real time RT-PCR
Ribonuclease protection assay (RPA)
RNAi/siRNA
RNA microinjection
RT-PCR (gel-based)
Sample preparation
None of the above
Contact Information:
First Name
Last Name
Title
Company/Institution
Dept/Bldg/Room No.
Address 1
Address 2
City
State/Province
ZIP/Postal Code
Country
Telephone
Fax
E-mail
In addition to the siRNA Screening Application Guide, I would like to receive:
Silencer--a monthly RNAi-focused email newsletter
RNA
FlashNotes--a general email newsletter, sent out monthly
TechNotes--a general print newsletter, mailed out 4 times per year
2007 Catalog