Post-translational silencing of gene expression is a powerful molecular tool for stem cell research to study gene function, biological pathways and physiology of disease. Gene silencing via siRNA enables several applications:

The Applied Biosystems siRNA Analysis Workflow is an optimized system for inducing gene silencing. Benefits include:

• Integrated workflow: Solutions for siRNA selection, transfection, validation and functional analysis by real-time PCR
• GeneAssist™ Pathway Atlas: An interactive on-line tool to select siRNAs and assays within the context of biological process
• Silencer® Select Pre-designed siRNAs: Chemically modified siRNAs with improved silencing efficacy, potency and specificity that minimize artifacts due to poor silencing and off-target effects

• Select siRNAs: Use GeneAssist™ Pathway Atlas, an interactive on-line tool to view and select gene or protein targets within the context of biological process, such as Nanog in ESC pluripotency. Choose Silencer® Select Pre-Designed siRNAs or Custom Designed Silencer® Select siRNAs, chemically modified siRNAs with improved silencing efficacy, potency and specificity that minimize artifacts due to poor silencing and off-target effects.
  
  
• Optimize Delivery: Optimize delivery: The siPORT™ Electroporation Kit or Silencer® siRNA Transfection Kit II include reagents and controls to determine optimal conditions for delivery by transfection or electroporation.
  
  
• Test Efficiency: Validate the siRNA targets using the TaqMan® Gene Expression Workflow.
  
  
• Examine Biologically: Assess the phenotypic effects by examining cell morphology, measuring mRNA with TaqMan® Gene Expression Workflow or protein levels with the Western-Star™ Immunodetection System.
  
  
• Correlate: Compare phenotype and level of gene knockdown using time course or siRNA titration studies.
  
  

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