An Optimized Gene Expression Workflow for Single Cells
Applied Biosystems scientists have improved the reliability of gene expression analysis in single cells by optimizing the protocol for the TaqMan® PreAmp Cells-to-CT™ Kit to prepare single cells for preamplification and real-time PCR analysis.
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Obtaining the Highest Quality RNA from Blood Samples

In this article we discuss these challenges and the methods available for isolating high quality RNA from blood.
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Variation of microRNA Profiles in Normal vs. Cancerous Hepatocytes

Applied Biosystems offers a complete portfolio of products and protocols for the discovery and profiling of miRNAs. Here, miRNA profiling tools are used to investigate miRNA differences in primary human hepatocytes compared to liver cancer cell lines.
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Tips from the Bench: Get Control of Your siRNA Experiments

Applied Biosystems scientists offer advice on the proper use of controls for siRNA experiments.
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Your Data: LCM Staining Effects on Real-Time RT-PCR

Research is described demonstrating that the Ambion® LCM Staining Kit can be successfully used on formalin-fixed and paraffin-embedded (FFPE) tissues without compromising either the yield or the quality of RNA extracted.
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Removing Viral RNA Improves Microarray Profiling of Virus-Infected Cells

Scientists report a method using a modified Ambion® GLOBINclear™ System to specifically deplete viral mRNA and increase the quality of microarray data from mixed host:virus RNA samples.
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Top Five Tips for Working with Limited Samples

Tip #3: Skip RNA Purification. One can now directly perform RT-PCR from cultured cells without first isolating RNA... View this and other tips by clicking the link below.
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Scientist Profile: An Interview with Dr. Junko Stevens

Learn more about leader and driving force in the development of our instruments and reagents for PCR and real-time PCR.
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