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TaqMan® Gene
Expression Cells-to-CT™ Kit
This kit enables researchers to easily perform expression analysis
of cultured cells
without purification of RNA. The kit includes reagents for cell
lysis and RNA preservation,
genomic DNA removal, and reverse transcription (RT). The kit also
includes TaqMan Gene Expression
Master Mix for use in subsequent real-time PCR analysis.
TaqMan® PreAmp
Cells-to-CT™ Kit
The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform
gene expression analysis directly from limited or small numbers
of cultured cells without RNA purification. The protocol includes
an intermediate amplification step (pre-amplification) between
reverse transcription and real-time PCR in which cDNA is enriched
for up to 100 gene targets of the user's choice without amplification
bias.
TaqMan® MicroRNA
Cells-to-CT™ Kit
The TaqMan MicroRNA Cells-to-CT Kit makes it possible to perform
microRNA (miRNA) expression profiling directly from cultured cells
without RNA purification. The kit is the first to offer a simple,
complete workflow for miRNA expression analysis from a few samples,
or it can easily be incorporated into automated, high throughput
applications.
TaqMan® Cells-to-CT™ Control
Kit
Designed for use with the TaqMan Gene Expression Cells-to-CT Kit,
this kit contains an endogenous control (ACTB) to normalize for
sample loading variability and an artifical XenoRNA™ control
and corresponding TaqMan Gene Expression Assay to monitor the effects
of PCR inhibition.
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