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Journal Club & RNArticles


A Role for a Mammalian miRNA in B cell Development
A review of: Chen C-Z, Li L, Lodish HF, Bartel DP (2004) MicroRNAs modulate hematopoietic lineage differentiation. Science 303: 83-6.

A Small RNA is Discovered that Activates Gene Expression at a Transcriptional Level
A review of: Kuwabara T, Hsieh J, Nakashima K, Taira K, Gage FH (2004) A small modulatory dsRNA specifies the fate of adult neural stem cells. Cell 116:779-93.

Antisense RNA and the Neurospora Circadian Clock
A review of Kramer C, Loros JL, Dunlap JC, and Crosthwaite SK (2003). Role of antisense RNA in regulating circadian clock function in Neurospora crassa. Nature421:948-952.

Argonaut4 is required for Transcriptional Gene Silencing (TGS)
A review of Zilberman D, Cao X, and Jacobsen SE. (2003). ARGONAUTE4 control of locus-specific siRNAs accumulation and DNA and histone methylation. Science 299:716-719.

Drosophila miRNA Functions Like an Oncogene
A review of Brennecke J, Hipfner DR, Stark A, Russell RB, and Cohen SM. (2003). bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell 113:25-36.

Expression of siRNAs from an Inducible Pol III Promoter
A review of vandeWetering M,Oving I,Muncan V,TjonPonFong M,Brantjes H,vanLeenen D,Holstege FCP, Brummelkamp TR,Agami R,Clevers H. (2003) Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector. Embo reports 4, 6:609-15.

Genome-wide RNAi Analysis in Worms: The Case of Fat Regulation
A review of Ashrafi K, Chang FY, Watts JL, Fraser AG, Kamath RS, Ahringer J, and Ruvkun G. (2003). Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes. Nature 421:268-272.

Lentiviral-Mediated siRNA Delivery into Primary T Cells
Review of Qin X-F, An DS, Chen ISY, and Baltimore D. (2003). Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. Proc Natl Acad Sci USA 100(1): 183-188.

miRNA Involvement in Asymmetric Gene Expression
In the 25 December 2003 issue of Nature, Johnston and Hobert show that in C. elegans a certain left/right asymmetry is controlled in part by a novel microRNA (miRNA) called lsy-6.

RNArticles: Localizing mRNA in Living Cells and RNA-guided Nucleotide Modification
A review of two articles, one by Bratu et al about a new method of mRNA localization and one by Decatur and Fournier regarding RNA modification by RNA-protein complexes (RNP's).

RNArticles: Keeping RNA Intact Prior to RNA Isolation - RNAlater®
Summaries of four research articles citing the use of RNAlater Tissue Storage Solution.

RNArticles: RNA Amplification Used to Develop New In Vitro HCMV Assay
Research is described where RNA is amplified from a small number of human cytomegalovirus (HCMV) infected CD34+ progenitor cells to compare the expression pattern with that of normal fibroblasts and infected bone marrow cells.

RNArticles: siRNAs Can Function as miRNAs
This article by Doench, Petersen, and Sharp presents the results of several experiments exploring the overlap in siRNA and miRNA pathways.

Tissue-specific Expression of siRNAs to produce "Knockdown Mice"
A review of Shinagawa T and Ishii S. (2003). Generation of Ski-knockdown mice by expressing a long double-strand RNA from an RNA polymerase II promoter. Genes & Dev. 17:1340-1345.

Using DNA Microarrays to Determine siRNA Specificity
A review of Chi J-T, Chang HY, Wang NN, Chang DS, Dunphy N, and Brown PO (2003). Genomewide view of gene silencing by small interfering RNAs. Proc. Natl. Acad. Sci. USA (early online edition, 2 May 2003).

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