Products Documents
Experimental Workflow:
Using siRNAs to Delineate Gene Function

All Products

Step 1. Obtain effective siRNAs

Silencer® Pre-designed siRNAs
Silencer® Validated siRNAs
Silencer® Positive Control siRNAs
Silencer® Negative Control siRNAs
Silencer® siRNA Libraries
Custom siRNA Design and Synthesis

Step 2. Optimize delivery

Transfection
siPORT™ NeoFX™ Transfection Agent
siPORT™ Amine Transfection Agent
Silencer® siRNA Transfection Kit II
KDalert™ Kit
Silencer® siRNA Starter Kit

Electroporation
siPORT™ Electroporation Buffer
siPORT™ Electroporation Kit

Step 3. Test silencing efficiency

RNA Isolation
PARIS™ Kit (Protein and RNA Isolation System)
RNAqueous®-4PCR Kit
MagMAX™-96 Total RNA Isolation Kit

qRT-PCR (Applied Biosystems)
TaqMan® Gene Expression Assays
TaqMan® Gene Expression Master Mix
High Capacity cDNA Reverse Transcription Kit
7900HT Fast Real-time PCR System
7500 Real-time PCR System

Step 4. Examine biological impact

Cell-Based Assays (Applied Biosystems)
8200 Cellular Detection System
Tropix® Cell Signaling and Reporter Gene Assays

Protein Detection
Western-Light™ Immunodetection System (Applied Biosystems)
Antibodies Matched to Silencer® Control siRNAs

qRT-PCR (Applied Biosystems)
TaqMan® Gene Expression Assays
TaqMan® Gene Expression Master Mix
High Capacity cDNA Reverse Transcription Kit
7900HT Fast Real-time PCR System
7500 Real-time PCR System

Step 5. Correlate silencing with biological impact

RNA Isolation
PARIS™ Kit (Protein and RNA Isolation System)
RNAqueous®-4PCR Kit
MagMAX™-96 Total RNA Isolation Kit

qRT-PCR (Applied Biosystems)
TaqMan® Gene Expression Assays
TaqMan® Gene Expression Master Mix
High Capacity cDNA Reverse Transcription Kit
7900HT Fast Real-time PCR System
7500 Real-time PCR System

Protein Detection
Western-Light™ Immunodetection System (Applied Biosystems)
Antibodies Matched to Silencer® Control siRNAs


NOTE: Most Ambion products can also be ordered through the Applied Biosystems website.

 

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