Products Documents
Experimental Workflow:
Using siRNAs to Delineate Gene Function
Recommended Products
Step 5. Correlate silencing with biological impact

RNA Isolation

PARIS™ Kit (Protein and RNA Isolation System)
Isolate both RNA and native protein from the same experimental sample.

RNAqueous®-4PCR Kit
Phenol-free total RNA isolation from >105 cultured cells or >10 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

MagMAX™-96 Total RNA Isolation Kit
Isolate total RNA from cultured mammalian cells (up to 105 cells), mammalian tissues (up to 10 mg), or even small plant samples in 96 well plates using magnetic bead-based separation technology.

qRT-PCR (Applied Biosystems)
TaqMan® Gene Expression Assays
TaqMan® Gene Expression Master Mix
High Capacity cDNA Reverse Transcription Kit
7900HT Fast Real-time PCR System
7500 Real-time PCR System

Protein Detection
Western-Light™ Immunodetection System (Applied Biosystems)
Antibodies Matched to Silencer® Control siRNAs


NOTE: Most Ambion products can also be ordered through the Applied Biosystems website.

 

     For Research Use Only. Not for use in diagnostic procedures.
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