Experimental Workflow:
Using siRNAs to Delineate Gene Function

Recommended Products

Step 4. Examine biological impact

Cell-Based Assays (Applied Biosystems)
8200 Cellular Detection System
Tropix® Cell Signaling and Reporter Gene Assays

Protein Detection
Western-Light™ Immunodetection System (Applied Biosystems)
Antibodies Matched to Silencer® Control siRNAs

qRT-PCR (Applied Biosystems)
TaqMan® Gene Expression Assays
TaqMan® Gene Expression Master Mix
High Capacity cDNA Reverse Transcription Kit
7900HT Fast Real-time PCR System
7500 Real-time PCR System

NOTE: Most Ambion products can also be ordered through the Applied Biosystems website.

For Research Use Only. Not for use in diagnostic procedures.

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