Experimental Workflow:
Using siRNAs
to Delineate Gene Function
Recommended Products
Step 2. Optimize delivery
Transfection
siPORT™ NeoFX™ Transfection
Agent
A lipid-based transfection agent that can be used to efficiently transfect adherent cells as they are subcultured - without increased cytotoxicity.
siPORT™ Amine Transfection
Agent A polyamine transfection agent optimized for the transfection of siRNA into a wide variety of cultured cells.
Silencer® siRNA
Transfection Kit II
Two different transfection agents packaged together with both a positive and negative control siRNA to facilitate optimization experiments.
KDalert™ Kit Obtain a reliable measure of GAPDH enzyme activity in cultured human, mouse, or rat cells in less than 30 minutes using a microplate fluorometer.
Silencer® siRNA
Starter Kit
All of the reagents, protocols, and instructions necessary to get underway with siRNA transfection and ensure successful gene knockdown experiments.
Electroporation
siPORT™ Electroporation
Buffer
For use with multiple types of electroporators to ensure highly efficient delivery of siRNA into cells while maintaining high cell viability.
siPORT™ Electroporation
Kit
Contains siRNA Electroporation Buffer, plus a CyT3-labeled control siRNA, GAPDH control siRNA, and negative control siRNA.
NOTE: Most Ambion products can also be ordered through the Applied Biosystems website.
For Research Use Only. Not for use in diagnostic procedures.