Experimental Workflow:
miRNA Expression Analysis

All Products

Step 1. Isolate miRNA

RNAlater® Solution
mirVana™ miRNA Isolation Kit
mirVana™ Probe & Marker Kit
Acrylamides
Urea
Gel Loading Solutions
Decade™ Markers
TBE Buffer (solution)
TBE Buffer (powder)

Step 2. Convert to cDNA

TaqMan® MicroRNA Reverse Transcription Kit (Applied Biosystems)
TaqMan® MicroRNA Assays (Applied Biosystems)

Step 3. Quantitate miRNA expression

TaqMan® MicroRNA Assays (Applied Biosystems)
TaqMan® Universal PCR Master Mix (Applied Biosystems)
Applied Biosystems 7900HT Fast Real-Time PCR System

What's next?

Anti-miR™ miRNA Inhibitors
Pre-miR™ miRNA Precursor Molecules

NOTE: Most Ambion products can also be ordered through the Applied Biosystems website.

Ambion and Applied Biosystems products are for Research Use Only, not for use in diagnostic procedures.