Products Documents
Experimental Workflow:
miRNA Expression Analysis
Recommended Products
Step 3. Quantitate miRNA expression

TaqMan® MicroRNA Assays (Applied Biosystems)
TaqMan MicroRNA Assays quantitate microRNAs with the specificity and sensitivity of TaqMan assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan probes. The assays target only mature microRNAs, not their precursors, ensuring biologically relevant results.

TaqMan® Universal PCR Master Mix (Applied Biosystems)
This kit contains components needed for all 5' nuclease assays using TaqMan®-probe PCR applications. This easy-to-use solution minimizes your assay set-up time and employs AmpliTaq Gold® DNA polymerase for enhanced performance.

Applied Biosystems 7900HT Fast Real-Time PCR System
The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading—and now also offers optional Fast real-time PCR capability.



NOTE: Most Ambion products can also be ordered through the Applied Biosystems website.



Ambion and Applied Biosystems products are for Research Use Only, not for use in diagnostic procedures.

 
 
 
 
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