Isolation of miRNA begins when total RNA that includes the small RNA fraction is isolated from the samples of interest. However, not all isolation methods retain the small RNA fraction...
Use the components of the TaqMan® MicroRNA Reverse Transcription Kit along with the RT primer provided with the chosen TaqMan MicroRNA Assay to convert miRNA to cDNA.
Functional Analysis--Subsequent steps in this workflow might include the examination of miRNA function by up and down regulating specific miRNA levels to study endogenous or reporter gene regulation and phenotypic response.
