Applied Biosystems guarantees that when you purchase two Silencer Select Pre-designed siRNAs to the same target, both will knock down target mRNA levels in cultured cells at least 70% when measured 48 hours after transfection at 5 nM or higher final siRNA concentration under the conditions described below. If both Silencer Select Pre-designed siRNAs do not induce >70% target mRNA knockdown, Applied Biosystems will provide a one-time replacement of up to two Silencer Select Pre-designed siRNAs per target at no additional charge. When you purchase three Silencer Select Pre-designed siRNAs to the same target, Applied Biosystems guarantees that at least two will knock down target mRNA levels in cultured cells at least 80% when measured 48 hours after transfection at 5 nM or higher final siRNA concentration under the conditions described below. If at least two of the three Silencer Select Pre-designed siRNAs do not induce >80% target mRNA knockdown, Applied Biosystems will provide a one-time replacement of up to two Silencer Select Pre-designed siRNAs per target at no additional charge. Requests for replacement product must be made within one hundred and eighty (180) days from the date of delivery of the Silencer Select Pre-designed siRNAs.

Optimum transfection efficiency must be confirmed using good laboratory practices and a proven-to-work siRNA to an endogenous message, such as Ambion Silencer Select GAPDH siRNA Control. To assess knockdown, target mRNA levels in treated samples must be compared to that of cells transfected with a nontargeting control siRNA, such as Silencer Select Negative Control #1. We recommend Applied Biosystems TaqMan® Gene Expression Assays to quantify mRNA levels.

This guarantee does not extend to any replacement product. PRODUCT REPLACEMENT AS SET FORTH ABOVE IS APPLIED BIOSYSTEMS’ SOLE OBLIGATION AND CUSTOMER’S SOLE AND EXCLUSIVE REMEDY WITH RESPECT TO THE GUARANTEE GIVEN ABOVE.

To arrange a replacement, contact Technical Services. Please be ready to supply data indicating transfection efficiency and measurement of mRNA knockdown.

Ambion guarantees that when you purchase three Silencer Pre-designed siRNAs to the same target, at least two of the siRNAs will reduce target mRNA levels by 70% or more when transfected under appropriate conditions at 100 nM and target mRNA levels are quantitated 48 hours later.

Appropriate transfection conditions should be confirmed using a validated, proven to work siRNA to an endogenous message, such as the Silencer GAPDH siRNA Control, and the extent of mRNA knockdown elicited should be compared to cells transfected with a nonsense control siRNA, such as the Silencer Negative Control #1. Silencing results should be normalized for transfection efficiency.

Ambion recommends the use of Applied Biosystems TaqMan® Gene Expression Assays to monitor effects on target RNA levels in response to an siRNA.

Applied Biosystems is a registered trademark of Applera Corporation. TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Each Silencer Select Validated siRNA is guaranteed to knock down target mRNA levels 80% or more in cultured cells when measured 48 hours after transfection at 5 nM or higher final siRNA concentration under the conditions described below. If a Silencer Select Validated siRNA does not induce target knockdown as described above, Applied Biosystems will provide a one-time replacement of another Silencer Select Validated siRNA, or, if none are available, a Silencer Select Pre-designed siRNA to the same target, at no additional charge. Requests for replacement product must be made within one hundred and eighty (180) days from the date of delivery of the Silencer Select Validated siRNA.

Optimum transfection efficiency must be confirmed using good laboratory practices and a proven-to-work siRNA to an endogenous message, such as Ambion Silencer Select GAPDH siRNA Control. To assess knockdown, target mRNA levels in treated samples must be compared to that of cells transfected with a nontargeting control siRNA, such as Silencer Select Negative Control #1. We recommend Applied Biosystems TaqMan® Gene Expression Assays to quantify mRNA levels.

To arrange a replacement, contact Technical Services. Please be ready to supply data indicating transfection efficiency and measurement of mRNA knockdown.

This guarantee does not extend to any replacement product. PRODUCT REPLACEMENT AS SET FORTH ABOVE IS APPLIED BIOSYSTEMS' SOLE OBLIGATION AND CUSTOMER'S SOLE AND EXCLUSIVE REMEDY WITH RESPECT TO THE GUARANTEE GIVEN ABOVE.

Ambion guarantees that each Silencer Validated siRNAs will reduce target mRNA levels by 70% or more when transfected under appropriate conditions at 100 nM and target mRNA levels are quantitated 48 hours later.

Appropriate transfection conditions should be confirmed using a validated, proven to work siRNA to an endogenous message, such as the Silencer GAPDH siRNA Control, and the extent of mRNA knockdown elicited should be compared to cells transfected with a nonsense control siRNA, such as the Silencer Negative Control #1. Silencing results should be normalized for transfection efficiency.

Applied Biosystems is a registered trademark of Applera Corporation. TaqMan is a registered trademark of Roche Molecular Systems, Inc.