• High yields of high specific activity, full-length RNA probes in just 10 minutes
  
  
• Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots and in situ hybridization
  
  
• Includes TURBO DNase™
  
  
• Kits available for DNA templates containing T7, T3, and SP6 promoters
  
  
• All kits contain quality tested DNase/RNase-free reagents
  
  

Ambion's MAXIscript In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 x 10⁹ cpm/µg in just 10 minutes. High specific activity, single-stranded RNA probes are used in ribonuclease protection assays, in situ hybridization, and blot hybridizations. MAXIscript Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2-6 µg/20µl reaction) of up to 2 kb.

In ribonuclease protection assays, it is essential to have high yields of full-length probe. However, maximizing sensitivity requires using high specific activity but low concentrations of the labeled nucleotide, which can greatly reduce yields of full-length transcripts. MAXIscript Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays.

The MAXIscript Kits are also an excellent choice for synthesis of nonisotopically labeled probes. The labeling can be accomplished post-transcriptionally by using Ambion's BrightStar® Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction.

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