• DNase treated to remove contaminating DNA
  
  
• Unsurpassed quality control
  
  
• Certified to contain small RNAs (miRNA, siRNA, and snRNA)
  
  

With the emergence of more sophisticated techniques for gene expression analysis (e.g. microarray analysis, real-time RT-PCR), the quality of the RNA samples being studied becomes increasingly critical. As The RNA Company^®, Ambion is committed to providing the highest quality RNA available. To achieve this goal it is necessary to understand what "high quality RNA" truly means with respect to various downstream applications. Intactness of mRNA, genomic DNA contamination, presence/absence of enzymatic inhibitors, and ribosomal RNA contamination in poly(A) preparations are all important aspects of RNA quality. As we continue to gain a better understanding of how these factors affect downstream applications, we will strive to ensure that our FirstChoice RNA products exceed the requirements for even the most stringent applications.

As many researchers know, it can be extremely difficult to obtain high quality RNA from human brain tissue. Not only does this unique tissue require optimized protocols for isolation of RNA, but it also requires special precautions during collection to preserve RNA integrity. Ambion has worked with a number of organizations to develop protocols to ensure the consistent yield of high quality RNA from this invaluable tissue.

Each FirstChoice Human Brain Region Total RNA is subjected to the same stringent Quality Control standards as our other FirstChoice RNAs. The samples are processed using Ambion's RNA isolation reagents to produce highly pure, intact RNA. A stringent DNase treatment is performed to ensure that the RNA is ready for use in any downstream application, including RT-PCR. Note: While it is impossible to remove every DNA molecule from an RNA sample, each preparation is tested to ensure that any residual DNA contamination is insignificant.

The integrity of the RNA is verified by capillary electrophoresis using the Agilent® 2100 bioanalyzer. FirstChoice Total RNA is provided in THE RNA Storage Solution at a convenient, ready-to-use concentration of 1 mg/ml.

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