• Customers provide their own primers and probes
  
  
• Consistently detect RNA targets with high specificity and sensitivity
  
  
• Simplified setup due to fewer components
  
  
• Obtain results in about 1 hour
  
  
• Contains ROX for quantitative fluorescent signal normalization
  
  

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification and detection of RNA targets using a rapid single-tube real-time reverse transcription PCR (RT-PCR) strategy. The kit was developed using real-time detection of reaction products via TaqMan® chemistry.

AgPath-ID One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and reducing setup time. Once assembled, results are available in approximately one hour. The RT-PCR Enzyme Mix included in the kit is composed of highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT capable of producing high cDNA yields, and AmpliTaq® Gold, the preferred hot-start DNA polymerase for specific target amplification. The included RT-PCR Buffer contains optimized reagents for efficient, robust reverse transcription and PCR; it also contains the passive reference dye, ROX, for quantitative fluorescent signal normalization. A Detection Enhancer is also provided as an optional reagent for amplification of templates with a high GC content or persistent secondary structure.


This product is compatible with the 5' nuclease detection and dsDNA-binding dye processes covered by patents owned or licensable by Applied Biosystems. No license under these patents is conveyed expressly, by implication, or by estoppel to the purchaser by the purchase of this product. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Trademarks, Patents, Licensing