• Choose from three different SlideHyb Glass Array Hybridization Buffers for consistent, superior results
  
  

An Array of Arrays
Many differences exist between the various commercial and lab-prepared glass arrays. The type of glass slide used, the length and GC content of the attached nucleic acid targets, and the manufacturing protocol employed all influence the optimal hybridization conditions for glass microarray analysis. With all the different variables to consider, it is impossible for one hybridization buffer protocol to be ideal for all glass slide array experiments. Our selection of SlideHyb™ Glass Array Hybridization Buffers allow you to determine the ideal conditions for your system.

The Right Hybridization Buffer
The SlideHyb Buffers have identical salt and formamide compositions resulting in similar hybridization stringencies. They vary in hybridization kinetics and blocking reagents. SlideHyb Buffer #1 is a basic buffer which typically exhibits the lowest background of the three buffers. SlideHyb Buffers #2 and #3 are both enhanced for ultrasensitivity but may exhibit higher background than #1 depending on the slide manufacturing method.

How to Customize Your Hybridization Protocol
When developing a glass array hybridization protocol, try multiple SlideHyb Buffers. Use SlideHyb Buffer #1 at various hybridization temperatures to determine the best temperature. Then, use that temperature to compare the various hybridization buffers. Choose the SlideHyb Buffer that gives the greatest signal and lowest background for your experiment.

Glass Array Hybridization Cassette
The Glass Array Hybridization Cassette is designed for glass microarray hybridization reactions.

Trademarks, Patents, Licensing