• A comprehensive probe set for microRNA expression profiling
  
  
• Targets the human, mouse, and rat miRNAs in miRBase 8.0, plus 150+ novel human miRNAs
  
  
• Use for the detection of differential miRNA expression across tissues and among different disease states
  
  
• Includes probes to new proprietary Ambi-miR™ miRNAs
  
  

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MicroRNAs (miRNAs) are evolutionarily conserved, small, non-coding RNA molecules that are estimated to regulate at least 30% of all protein encoding genes. Currently, more than 2,900 miRNAs have been annotated from a wide range of organisms, including plants, worms, flies, mammals, and viruses. To date, miRNAs have been implicated in development, differentiation, apoptosis, fat metabolism, viral infection, and cancer. Evaluation of the global expression of miRNAs will likely identify regulation points for many different biological processes.

mirVana miRNA Probe Set

Probe Sets	Number
Human	328
Mouse	266 (114)*
Rat	238 (46)**
Ambi-miR™	152
Controls	22
Total	662
*Unique probes that are not human are in parentheses. **Unique probes that are not human or mouse are in parentheses.

The mirVana miRNA Probe Set (patent pending) is a collection of amine-modified DNA oligonucleotides targeting a comprehensive selection of human, mouse, and rat miRNAs from miRBase Sequence Database version 8.0. In addition, the mirVana miRNA Probe Set includes probes targeting an exclusive set of newly identified human miRNAs. Called Ambi-miR™ miRNAs, these novel miRNAs were identified through a combination of bioinformatic prediction, cloning, and detection in human total RNA samples. Developed in conjunction with the mirVana™ miRNA Labeling Kit, the mirVana miRNA Probe Set is designed for preparation of glass slide miRNA arrays. Process the array using components of the mirVana™ Array System (see Figure 2) then analyze using commercially available microarray analysis instruments and software to obtain global miRNA expression profiles.

The probes in the mirVana miRNA Probe Set are 42–46 nucleotides (nt) long and consist of an 18–24 nt segment that targets a specific known human, mouse, or rat miRNA, plus spacer and attachment sequences for coupling to the surface of the microarray slide. The probes are synthesized with an amine-modification for compatibility with both epoxy and aldehyde slide surface attachment chemistries. We recommend SCHOTT Nexterion® Slide E microarray slides as they are convenient, easy to use, and provide consistent results. However, the mirVana miRNA Probe Set is compatible with all common array attachment chemistries except 3-D gel matrix slides. (Gel matrix slides are incompatible with the spacer and linker sequences within the miRNA Probe Set oligonucleotides.)

Contents
The mirVana miRNA Probe Set is provided in 96 well plates; each well contains 500 pmol of dried amine-modified oligonucleotide. Detergent concentrate, salt concentrate, and 3X hybridization buffer are included with the Probe Set. (The hybridization buffer is also available separately.) A complete listing of the probes in the probe set can be found here:
>> Probe List (Excel)

NOTE: The purchase of Ambion products does not convey any license under any patent rights owned by Oxford Gene Technology IP Limited (“OGT”) including any right to make or use oligonucleotide arrays under OGT’s patents, i.e. US, European, Japanese patents and patent applications deriving from WO 89/10977, WO 90/ 03382 and WO 96/31622. Please contact OGT to enquire about a license under OGT’s patents at licensing@ ogt.co.uk. Further details can be found at www.ogt.co.uk.

Trademarks, Patents, Licensing

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mirVana™ miRNA Isolation Kit mirVana™ miRNA Reference Panel Anti-miR™ miRNA Inhibitors pMIR-REPORT™ miRNA Expression Reporter Vector System Pre-miR™ miRNA Precursor Molecules Pre-miR™ miRNA Starter Kit RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE Tissues TaqMan® MicroRNA Cells-to-CT™ Kit