• Removes common plant contaminants such as polyphenolics and polysaccharides
  
  
• No phenol extraction, alcohol precipitation or proteinase digestion steps required when used with RNAqueous® kit
  
  

Ambion's Plant RNA Isolation Aid is recommended for use with the RNAqueous® Kit for purification of total RNA from plant tissues. The reagent contains polyvinylpyrrolidone (PVP), a high molecular weight polymer that binds to contaminants such as polyphenolics and polysaccharides that are commonly present in plant tissues.

Glass fiber filter-based methods of isolation, such as the RNAqueous Kit, are especially suited for plant RNA isolation because it does not include any alcohol precipitation steps; many common contaminants found in plant tissue are known to co-precipitate with nucleic acid during alcohol precipitation. When used with the RNAqueous Kit, the procedure consists of disrupting the plant tissue in a guanidinium-based lysis solution to which the Plant RNA Isolation Aid has been added. A brief centrifugation then removes the polyphenolic and polysaccharide contaminants. The supernatant is passed through a glass fiber filter under conditions that support RNA binding to glass. The filter is then washed to remove other contaminants and the RNA is eluted in a small volume (50 µl or more) of elution solution.

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