All Ambion Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity and for functionality. These are the same solutions we use in-house and in our kits.

Gel Loading Buffer II - Denaturing PAGE
A 1-2X solution of 95% Formamide, 18mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue.

Gel Loading Solution - All purpose, native agarose
A 10X solution of 40% Sucrose, 0.17% Xylene Cyanol,and 0.17% Bromophenol Blue.

NorthernMax Formaldehyde Load Dye
This ready-to-use solution is added to RNA sample (3 parts solution : 1 part sample) and heated briefly. The samples are then ready to be loaded. Ethidium bromide can be added to the samples if desired.

NorthernMax-Gly Sample Loading Dye
An improved formulation used for RNA sample denaturation in any glyoxal gel protocol. The volume ratio of solution to sample is lower than in published protocols, so sample precipitation prior to gel loading is usually not required. Ethidium bromide is premixed into the solution.

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