- No post-treatment autoclaving
- Safe, easy-to-use reagent inactivates RNases in solution
- Inactivation process can be repeated to protect against newly introduced contaminants
- Compatible with downstream procedures, such as RT-PCR and in vitro transcription
- Inactivates RNases in Tris and other solutions that cannot be treated with DEPC
- Supplied at a working concentration for direct resuspension of RNA pellets
RNAsecure™ Resuspension Solution contains the same active ingredients as the RNAsecure Reagent, but is supplied at working concentration for direct resuspension of RNA pellets. It is specifically meant for resuspending RNA pellets that may contain trace amounts of ribonuclease (e.g., after total RNA isolation). Note that RNAsecure cannot reverse RNA degradation that has already occurred, but can prevent further RNA degradation.
To use, the RNA pellet is resuspended in the RNAsecure Resuspension Solution and heated to 60°C for 10 minutes. Like the RNAsecure Reagent, the RNAsecure Resuspension Solution can be reheated to eliminate any RNAse contamination introduced at a later time.
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