• These nontargeting siRNAs have limited sequence similarity to known genes
  
  
• Validated for use in human, mouse, and rat cells
  
  
• Functionally proven to have minimal effects on cell proliferation and viability
  
  
• Available individually and as a panel of 7 negative control siRNAs
  
  
• HPLC purified, duplexed, and ready to use
  
  

SEE RELATED PRODUCTS

See also: Silencer® Select Positive & Negative Control siRNAs Silencer® Positive Control siRNAs Silencer® Labeled Control siRNAs

Important Licensing Information

Negative Controls
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining transfection efficiency and to control for the effects of siRNA delivery. In siRNA screening applications, negative control siRNAs are also critical for setting the "hit" threshold that determines whether an siRNA is considered to have a positive, negative, or neutral effect in a particular assay.

Ambion has designed and tested seven Negative Control siRNAs that have no significant sequence similarity to mouse, rat, or human gene sequences. They have all been tested in cell-based screens and proved to have no significant effect on cell proliferation, viability, or morphology. Negative Controls #1 and #2, our most popular negative control siRNAs, are available in 40 nmol and 5 x 40 nmol sizes, in addition to the standard 5 nmol size.

In Vivo Ready Negative Control
Our "In Vivo Ready" Silencer Negative Control #1 siRNA is subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 µM in presence of deionized water, in vivo Ready siRNAs contain <0.6 mM Na+, <2.0 mM K+, and <0.1 mM Mg2+.

Silencer siRNA Screening Control Panel
The Silencer siRNA Screening Control Panel, which includes all seven negative control siRNAs, is offered for those researchers performing siRNA screens that employ hundreds or thousands of siRNAs per experiment. Because of the importance of such screens and the fact that some negative control siRNAs may have unintended effects in a particular assay, most researchers carefully test their negative control siRNAs before choosing 2–6 control siRNAs for inclusion in their siRNA library screen. The Silencer siRNA Screening Control Panel simplifies this process. As an added benefit, a positive control siRNA to human, mouse, and rat KIF11 (Eg5) is included in the panel. Knockdown of KIF11, which encodes a kinesin family motor protein, leads to mitotic arrest. So effective delivery of KIF11 siRNA can be assessed visually; it can also be measured by several cell based assays.

The Silencer siRNA Screening Control Panel contains 1 nmol of each of the seven negative control siRNAs plus the KIF11 siRNA in a dried format. Interested in larger amounts of any Negative Control siRNA? Contact us at custom_services@ambion.com for a quote.

Trademarks, Patents, Licensing

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Silencer® Labeled Control siRNAs Silencer® Positive Control siRNAs Silencer® siRNA Libraries KDalert™ GAPDH Assay Kit Applied Biosystems: TaqMan® Gene Expression Assays