- Validated siRNA controls for optimizing siRNA experiments
- Gene-specific control siRNAs provided with scrambled, negative controls
- Functionally tested in several common cell lines
- HPLC purified, duplexed, and ready to use
Important Licensing Information
Premade, Gene Specific siRNA Controls
Silencer Control siRNAs, which target mRNAs frequently used as internal controls in RT-PCR, Northern blot, RPA, and other experiments designed to monitor gene expression, are ideal for developing and optimizing siRNA experiments. Each Silencer Control siRNA is validated for use in human cell lines, including the GAPDH siRNA optimized for mouse and rat. For measuring silencing at the protein level, a selection of antibodies are available, including antibodies against ß-actin and GAPDH. Each Silencer Control siRNA contains 5 nmol of ready-to-use chemically synthesized siRNA. A scrambled negative control siRNA (2 nmol) is included with most of these siRNAs. The popular Silencer GAPDH siRNAs are also available in 40 nmol and 5 x 40 nmol sizes.
In Vivo Ready Positive Control
Our "In Vivo Ready" Silencer GAPDH Positive Control siRNA is subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 µM in presence of deionized water, in vivo Ready siRNAs contain <0.6 mM Na+, <2.0 mM K+, and <0.1 mM Mg2+.
Trademarks, Patents, Licensing
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RELATED DOCUMENTS
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Specification Sheets
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Silencer® GAPDH siRNA (Human) (40 nmol)
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Silencer® GAPDH siRNA (Human) (5 nmol)
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Silencer® GAPDH siRNA (Human, Mouse, Rat) (40 nmol)
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Silencer® GAPDH siRNA (Human, Mouse, Rat) (5 nmol)
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Silencer® GFP (eGFP) siRNA
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Silencer® KIF11 (Eg5) siRNA (Human, Mouse, Rat)
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Silencer® GAPDH Positive Control siRNA, In Vivo Ready
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Citations
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MSDSs
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Supplemental Product Info.
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Phenotypes Induced by Successful Delivery of Silencer® KIF11 (Eg5) siRNA into Various Cell Lines
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TechNotes Articles
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Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
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RNAi: The Controls You Need
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Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect
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Resource Pages
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RNA Interference Resource
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Resource Pages (Product)
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siRNA Products & Services
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