SuperTaq™
This high purity Taq Polymerase is isolated from an E. coli strain that over-expresses this gene. Its heat stability allows DNA polymerization in the 5' to 3' direction during repeated heat denaturation and reannealing steps in the presence of dNTPs, a DNA template and complementary primers. SuperTaq™ Polymerase can be used in PCR and RT-PCR reactions, and has been optimized for use with Ambion's RETROscript™ First-Strand Synthesis Kit.

SuperTaq™ Plus
This extended range Taq Polymerase contains a proofreading activity that reduces the error rate of Taq Polymerase. SuperTaq Plus produces significantly higher yields of PCR products than ordinary Taq Polymerase, especially for fragments >1 kb, and can amplify up to 20 kb. SuperTaq Plus is suitable as a direct replacement for ordinary Taq Polymerase in most applications.

Unit Definition: One unit of SuperTaq or SuperTaq Plus incorporates 10 nmol of deoxynucleotides into acid insoluble material in 30 min at 74°C.

SuperTaq™ & SuperTaq™ Plus Supplied With:

dNTPs (2.5 mM each)
50 U size: 0.2 ml
250 U size: 1 ml

10X Reaction Buffer + MgCl₂ (15 mM)
50 U size: 1.25 ml
250 U size: 1.25 ml

10X Reaction Buffer - MgCl₂
50 U size: 1.25 ml
250 U size: 1.25 ml

MgCl₂ (25 mM)
50 U size: 1 ml
250 U size: 1 ml

SuperTaq and SuperTaq Plus are trademarks of and are manufactured by Enzyme Technologies Ltd. and sold under licensing arrangements with F. Hoffman-La Roche, Ltd., Roche Molecular Systems, Inc. and the Perkin-Elmer Corporation.

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