M-MLV RT Engineered to Maximize Full-length cDNA Synthesis
Reverse transcriptases (RTs) are used in a variety of molecular biology experiments, including cDNA library construction, RLM-RACE, and RNA amplification. A primary concern for many of these applications is the yield of full-length cDNA synthesized during the reverse transcription reaction. Use of specific reverse transcriptases that deliver high yields of full-length cDNA results in cDNA libraries that more faithfully represent the cellular transcriptome. Likewise, in RNA amplification reactions, high yields of full-length cDNA lead to high 5'/3' ratios, thus ensuring good quality microarray data.

ArrayScript is a M-MLV reverse transcriptase that has been engineered to produce high yields of full-length cDNA (Figure 1). In RNA amplification experiments, up to twice as much cRNA is synthesized when the first-strand cDNA is generated with ArrayScript compared to wild type M-MLV, AMV, or other engineered M-MLV reverse transcriptases when low amounts of total RNA (~100 ng) are used (Figure 2). This can eliminate the need to perform two rounds of cRNA amplification when working with limiting amounts of total RNA.

ArrayScript is also suitable for other RT and RT-PCR applications where high yields of full-length cDNA are required, such as cDNA library construction, 5' RLM-RACE, etc.

ArrayScript is provided in two convenient sizes: 4,000 U and 10,000 U (concentration = 200 U/µl). A 10X Reaction Buffer is supplied with the enzyme.

This enzyme is a component of the MessageAmp II aRNA Amplification Kit.

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