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NorthernMax® Kit

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Cat#
Product Name
Size
AM1940 NorthernMax® Kit 1000 cm2
AM10100 BrightStar®-Plus Positively Charged Nylon Membrane (5 membranes) 15 x 15 cm
AM10104 BrightStar®-Plus Positively Charged Nylon Membrane (large roll) 30 cm x 3 m
AM10102 BrightStar®-Plus Positively Charged Nylon Membrane (small roll) 30 x 45 cm
For Research Use Only. Not for use in diagnostic procedures.
  • Increase sensitivity up to 100X over standard hybridization protocols

  • Reduce hybridization to just 2 hours for many messages

  • Complete Northern Kit contains ULTRAhyb® Hybridization Buffer and gel and wash reagents for 10-20 gels

  • Compatible with RNA, DNA or oligonucleotide probes - labeled isotopically or nonisotopically

The NorthernMax Kit contains a complete set of RNase-free reagents for running formaldehyde-based Northern analysis. This kit is ideal for the investigator who wants to use familiar reagents while taking advantage of NorthernMax’s high sensitivity. The included ULTRAhyb® Ultrasensitive Hybridization Solution increases the sensitivity of RNA probes up to 20X and DNA probes up to 100X. Hybridization can be completed in just 2 hours for many messages. The NorthernMax Kit is compatible with DNA , RNA or oligo probes labeled isotopically or nonisotopically.

Each NorthernMax Kit provides a complete system. NorthernMax Kits contain everything you need to conduct Northerns, including high quality agarose, gel loading solution, gel buffer, running buffer, transfer buffer, ULTRAhyb Ultrasensitive Hybridization Buffer, wash solutions, and even RNaseZap®, a nontoxic ribonuclease decontamination solution for equipment and work surfaces. Positively charged nylon membranes must be purchased separately. Although the NorthernMax Kits are optimized for use with Ambion's BrightStar membrane, any positively charged nylon membrane is compatible. An RNA sample and template for probe synthesis are also provided as controls for each kit. NorthernMax kits can be used with either radiolabeled or nonisotopically labeled RNA or DNA probes.

Biocompare® Product Review

See Also:
NorthernMax® Northern Blotting Kits
Reagents for Northern Blotting
Strip-EZ™ StripAble™ Probe Synthesis and Removal Kits

Trademarks, Patents, Licensing

RELATED PRODUCTS
BrightStar®-Plus Positively Charged Nylon Membrane
ULTRAhyb® and ULTRAhyb-Oligo

RELATED DOCUMENTS
Protocols and Manuals
BrightStar®-Plus Nylon Membrane Protocol [go]
NorthernMax® Kit Instruction Manual [go]
Specification Sheets
BrightStar®-Plus Positively Charged Nylon Membrane [go]
Citations
MSDSs
Tools & Calculators
RNA and DNA Concentration Converter [go]
Molecular Biology Tables
Ribosomal RNA Sizes [go]
Supplemental Product Info.
Probe Labeling Chart [go]
General Articles
Northern Analysis: The Basics [go]
Technical Bulletins
Practical Tips for Optimal Northern Analysis [go]
Figures
[full-size]

Northern Analysis with Ambion Kits.

[full-size]

Sensitivity of Psoralen-Biotin, Enzymatic Biotin and 32P-labeled Probes in a Northern Blot.

[full-size]

Comparison of NorthernMax™ vs. Standard (Maniatis) Northern Blots.

 
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